Changes in glycine immunoreactivity in the rat superior olivary complex following deafness
Changes in glycine immunoreactivity in the rat superior olivary complex following deafness
复制标题
DOI：
10.1002/cne.20795
复制
发表时间：
2006-01-01
影响因子：
2.5
通讯作者：
Altschuler, RA
中科院分区：
文献类型：
--
作者：
Buras, ED;Holt, AG;Altschuler, RA
抑制性和兴奋性氨基酸神经递质之间的平衡有助于控制听性脑干的正常功能。听觉脑干通路中神经元活动水平的变化会影响抑制和兴奋之间的平衡。听觉通路中依赖活动的可塑性可以通过外周耳聋造成活动的大幅减少来研究。与耳聋相关的GABA的下降以前在下丘已经显示出来。然而，甘氨酸是成熟的上橄榄复合体(SOC)中更普遍的抑制递质。因此，本研究使用包埋后免疫细胞化学检测了SOC中是否存在与耳聋相关的甘氨酸变化。动物双侧耳廓内注射新霉素致聋。耳聋后14天，对耳聋大鼠SOC的外侧上橄榄核(LSO)、上橄榄旁核(SPON)、斜方体体内侧核、外侧核和腹侧核(MNTB、LNTB和VNTB)进行了检测，并与听力年龄匹配的正常对照组进行了比较。LSO和SPON可分为高频区和低频区。主细胞胞体上甘氨酸免疫反应阳性斑点的数量在所评估的所有区域都有显著减少，变化范围从VNTB的50%到LSO的23%不等。
The balance between inhibitory and excitatory amino acid neurotransmitters contributes to the control of normal functioning of the auditory brainstem. Changes in the level of neuronal activity within the auditory brainstem pathways influence the balance between inhibition and excitation. Activity-dependent plasticity in the auditory pathways can be studied by creating a large decrease in activity through peripheral deafening. Deafness-related decreases in GABA have previously been shown in the inferior colliculus. However, glycine is a more prevalent inhibitory transmitter in the mature superior olivary complex (SOC). The present study therefore examined if there were deafness-related changes in glycine in the SOC using postembedding immunocytochemistry. Animals were bilaterally deafened by an intrascalar injection of neomycin. Five nuclei in the SOC, the lateral superior olive (LSO), superior paraolivary nucleus (SPoN), and the medial, lateral, and ventral nuclei of the trapezoid body (MNTB, LNTB, and VNTB) were examined 14 days following the deafening and compared to normal hearing age-matched controls. The LSO and SPoN were divided into high and low frequency regions. The number of glycine immunoreactive puncta on the somata of principal cells showed significant decreases in all regions assessed, with changes ranging from 50% in the VNTB to 23% in the LSO.