Antifungal activity of camptothecin, trifolin, and hyperoside isolated from Camptotheca acuminata
Antifungal activity of camptothecin, trifolin, and hyperoside isolated from Camptotheca acuminata
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DOI：
10.1021/jf0484780
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发表时间：
2005-01-12
影响因子：
6.1
通讯作者：
Taylor, J
中科院分区：
文献类型：
--
作者：
Li, SY;Zhang, ZZ;Taylor, J
喜树碱是一种很有前途的抗肿瘤和抗病毒生物碱，但由于其生长受到限制，导致喜树的叶斑病和根腐病成为喜树的主要病害。生物测定表明，喜树碱纯品和从喜树中分离的黄酮类化合物（三叶草苷和金丝桃苷）在体外有效地控制真菌病原物，包括链格孢（Alternaria alternata）、黑附球菌（Epicoccum nigrum）、盖氏盘多毛孢（Pestalotia guepinii）、Drechslera sp.、和燕麦镰刀菌（Fusarium avenaceum），尽管这些化合物在植物中的抗真菌活性有限。CPT在10-30 μ g/mL时抑制菌丝体生长约50%（EC 50），在75-125 μ g/mL时完全抑制生长。黄酮类化合物在50 μ g/mL时的效果不如CPT，特别是在治疗后20天内，但在100或150 μ g/mL时更有效。CPT、三叶草苷和金丝桃苷可作为开发杀菌剂的先导。
Leaf spots and root rots are major fungal diseases in Camptotheca acuminata that limit cultivation of the plant for camptothecin (CPT), a promising anticancer and antiviral alkaloid. Bioassays showed that pure CPT and flavonoids (trifolin and hyperoside) isolated from Camptotheca effectively control fungal pathogens in vitro, including Alternaria alternata, Epicoccum nigrum, Pestalotia guepinii, Drechslera sp., and Fusarium avenaceum, although antifungal activity of these compounds in the plant is limited. CPT inhibited mycelial growth by approximately 50% (EC50) at 10-30 mug/mL and fully inhibited growth at 75-125 mug/mL. The flavonoids were less effective than CPT at 50 mug/mL, particularly within 20 days after treatment, but more effective at 100 or 150 mug/mL. CPT, trifolin, and hyperoside may serve as leads for the development of fungicides.