Effects of acute fasting and age on leptin and peroxisome proliferator-activated receptor gamma production relative to fat depot in immature and mature pigs.
Effects of acute fasting and age on leptin and peroxisome proliferator-activated receptor gamma production relative to fat depot in immature and mature pigs.
复制标题
急性禁食和年龄对未成熟和成熟猪中相对于脂肪库的瘦素和过氧化物酶体增殖物激活受体γ产生的影响。
DOI：
10.1111/j.1439-0396.2009.00968.x
复制
发表时间：
2010
影响因子：
2.7
通讯作者：
Garcia,MR
中科院分区：
文献类型：
--
作者：
O'Gorman,CW;Stanko,RL;Keisler,DH;Garcia,MR
Leptin and peroxisome proliferator‐activated receptor gamma (PPARγ) are adipogenic proteins that are actively involved in metabolic homeostasis of fat. Recently, it was reported that fat tissue in humans and rodents differs in metabolic activity relative to anatomical location of the fat tissue (i.e. depots) and animal age. Hence, we hypothesized that leptin and PPARγ production in various fat depots in female pigs differs in response to acute fasting, and that these responses vary with physiological maturity of the animal. Sixteen intact crossbred immature female pigs [prepubertal (PP); 132.2 ± 4.1 days] and 16 sexually mature female pigs (M; 224 ± 7.4 days) housed in an open‐air, concrete slab, sheltered barn were randomly assigned to either Control or Fasted treatments. Control pigs (PP,n= 8; M,n= 8) hadad libitumaccess to feed, while Fasted pigs (PP,n= 8; M,n= 8) were denied access to feed from the onset of the study (0 h) to euthanasia at 72 h. Immediately post‐mortem, fat samples were collected from the subcutaneous, pelvic, kidney, and heart (M pigs only) fat depots and analysed for leptin and PPARγ mRNA and protein content. Acute fasting decreased mean leptin mRNA tissue content in a depot specific manner in M pigs (p < 0.01), while mean leptin protein concentrations in fat tissues did not differ with fat depot or age of the pig. Furthermore, acute fasting did not affect mean PPARγ mRNA tissue content in a fat depot or age dependent manner. Mean concentrations of PPARγ protein in fat depots tended to be greater in M vs. PP pigs (p = 0.07). We suggest that these data provide evidence that acute fasting has a greater effect on leptin than PPARγ production in a fat depot dependent manner in M pigs, which may be indicative of changing physiological demands as an animal matures.
