pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface
pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface
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DOI：
10.1159/000454781
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发表时间：
2017-01-01
期刊：
影响因子：
4.2
通讯作者：
Takahashi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Mayanagi, Gen;Igarashi, Koei;Takahashi, Nobuhiro
评价牙齿表面/细菌界面的理化过程对于阐明龋齿的病因学是重要的。本研究旨在比较冠状面釉质（CE）和根面牙本质（RD）的矿物溶解性和蛋白质降解，并利用牙齿/细菌界面模型研究溶解组分在细菌引起的pH变化中的参与。形成井的实验装置由聚甲基丙烯酸甲酯制成，牛牙（CE或RD）样本固定在井的底部。将微型pH电极置于牙齿上，并将在含0.5%葡萄糖的复合培养基中生长的变形链球菌NCTC 10449细胞装入孔中。牙齿的pH值/S。在37 ℃下加入0.5%葡萄糖后，连续监测变形杆菌界面120分钟。S.从威尔斯孔中回收变形细胞，分别使用便携式乳酸盐计和荧光染料测量乳酸盐和钙的量。还通过荧光测定法评价蛋白水解活性。RD/S的pH值。mutans界面的表达明显高于CE/S。变形蛋白界面（30分钟：6.37 +/- 0.12对比6.18 +/- 0.11，60分钟：6.08 +/- 0.14对比5.66 +/- 0.27，90分钟：5.49 +/- 0.24对比5.14 +/- 0.22，p < 0.05）。RD（3.19 +/- 0.74 g/mL）中溶出的钙量大于CE（1.84 +/- 0.68 g/mL; p < 0.05），而产生的乳酸盐量相似。蛋白水解活性没有检测到在任何接口。这些结果表明RD比CE更易溶于细菌诱导的酸化。该方法有助于防龋材料的评价和开发。(C)2017 S. Karger AG，巴塞尔
Evaluating the physiochemical processes at the tooth surface/bacteria interface is important for elucidating the etiology of dental caries. This study aimed to compare the mineral solubility and protein degradation of coronal enamel (CE) and root dentin (RD), and investigate the involvement of dissolved components in bacteria-induced pH changes using a model of tooth/bacteria interface. An experimental apparatus forming a well was made of polymethyl methacrylate, and a bovine tooth (CE or RD) specimen was fixed at the bottom of the well. A miniature pH electrode was placed on the tooth, and Streptococcus mutans NCTC 10449 cells, grown in 0.5% glucose-containing complex medium, were packed into the well. The pH at the tooth/S. mutans interface was monitored continuously for 120 min after the addition of 0.5% glucose at 37 degrees C. S. mutans cells were recovered from the wells, and the amounts of lactate and calcium were measured using a portable lactate meter and a fluorescent dye, respectively. Proteolytic activity was also evaluated fluorometrically. The pH of the RD/S. mutans interface was significantly higher than that of the CE/S. mutans interface (30 min: 6.37 +/- 0.12 vs. 6.18 +/- 0.11, 60 min: 6.08 +/- 0.14 vs. 5.66 +/- 0.27, 90 min: 5.49 +/- 0.24 vs. 5.14 +/- 0.22, p < 0.05). Greater amounts of calcium were dissolved from RD (3.19 +/- 0.74 g/mL) than from CE (1.84 +/- 0.68 g/mL; p < 0.05), while similar amounts of lactate were produced. Proteolytic activity was not detected at any of the interfaces. These results indicate that RD is more soluble to bacteria-induced acidification than CE. This method can contribute to the evaluation and development of caries-preventive materials. (C) 2017 S. Karger AG, Basel