POSSIBLE MECHANISM OF REGULATING ADENYLATE-CYCLASE ACTIVITY IN ADIPOCYTE MEMBRANES FROM EXERCISE-TRAINED MALE-RATS
POSSIBLE MECHANISM OF REGULATING ADENYLATE-CYCLASE ACTIVITY IN ADIPOCYTE MEMBRANES FROM EXERCISE-TRAINED MALE-RATS
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DOI：
10.1016/s0006-291x(88)80502-3
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发表时间：
1988-03-30
影响因子：
3.1
通讯作者：
KOSHIMIZU, E
中科院分区：
文献类型：
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作者：
IZAWA, T;KOMABAYASHI, T;KOSHIMIZU, E
（-）-异丙肾上腺素刺激腺苷酸环化酶的活动显着更大的膜运动训练的雄性大鼠比久坐的雄性大鼠。在静止的膜中观察到毛喉素（10 μ M）刺激的环化酶活性的GTP抑制，而在来自训练的大鼠脂肪细胞的膜中GTP的抑制作用显著降低。用胰岛活化蛋白（一种百日咳毒素）处理膜，完全消除了两组之间毛喉素刺激的环化酶活性的GTP-甘氨酸差异。训练大鼠的膜中抑制性调节蛋白（41 kDa/40 kDa多肽）的含量比静止大鼠的膜低约40%，而刺激性调节蛋白（45 kDa多肽）的含量相当。它的结论是，脂肪细胞膜的环化酶活性的增强训练的雄性大鼠出现的结果，在一定程度上，由于抑制性调节蛋白的量的特定减少的抑制途径的衰减。
(-)-Isoproterenol-stimulated adenylate cyclase activities were significantly greater in membranes from exercise-trained male rats than in sedentary male rats. GTP-inhibition of forskolin (10 .mu.M)-stimulated cyclase activities were observed in sedentary membranes, whereas the inhibitory actions of GTP were significantly reduced in membranes from trained rat adipocytes. Treatment of membranes with islet-activating protein, a pertussis toxin, completely abolished the differences in GTP-inhibitoin of forskolin-stimulated cyclase activities between the two groups. The amounts of the inhibitory regulatory protein (41kDa/40kDa polypeptides) were about 40% less in membranes from trained rats than in sedentary membranes, wehreas that of the stimulatory regulatory protein (a 45kDa polypeptide) was equivalent. It is concluded that the enhanced cyclase activities of adipocyte membranes form trained male rats appear to result from, in part, an attenuation of the inhibitory pathway due to a specific decrease in the amount of the inhibitory regulatory proteins.