Distribution of Prolyl Oligopeptidase in Human Peripheral Tissues and in Ovarian and Colorectal Tumors
Distribution of Prolyl Oligopeptidase in Human Peripheral Tissues and in Ovarian and Colorectal Tumors
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DOI：
10.1369/0022155412453051
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发表时间：
2012-09-01
影响因子：
3.2
通讯作者：
Carpen, Olli
中科院分区：
文献类型：
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作者：
Myohanen, Timo T.;Pyykko, Elisa;Carpen, Olli
脯氨酰寡肽酶(PREP)是一种能水解短于30聚体的多肽的丝氨酸蛋白酶，与多种生理和病理条件有关。PREP主要是在大脑中研究的，但在外周组织中也检测到了显著的PREP活性。此外，在肿瘤中发现了更多的PREP活性。在这项研究中，作者研究了PREP蛋白在人外周组织以及卵巢和结直肠肿瘤中的免疫组织化学分布。研究发现，PREP广泛分布于人的外周组织，尤其是某些细胞。PREP在睾丸、卵巢、肝脏和皮肤的某些部位表达最强。在细胞水平上，高PREP水平被认为是分泌上皮细胞和参与繁殖的细胞的一种规则。在所研究的大多数肿瘤中，PREP的表达增加。PREP在卵巢上皮性肿瘤中的表达高于良性肿瘤，在卵巢上皮性癌中，随着恶性程度的升高，PREP的表达有增加的趋势。结果表明，PREP不仅与生殖有关，而且与分泌过程有关。PREP在恶性肿瘤中的表达高于良性肿瘤，提示PREP可能与肿瘤的扩张和转移有关。(《组织化学细胞化学杂志》60：706-715,2012)
Prolyl oligopeptidase (PREP) is a serine protease that hydrolyzes peptides shorter than 30-mer, and it has been connected with multiple physiological and pathological conditions. PREP has been mostly studied in the brain, but significant PREP activities have been measured in peripheral tissues. Moreover, increased PREP activities have been found in tumors. In this study, the authors studied the immunohistochemical distribution of PREP protein in human peripheral tissues and in ovarian and colorectal tumors. PREP was found to be widely distributed in human peripheral tissues and specifically in certain cells. The most intense PREP expression was seen in the testis, ovaries, liver, and some parts of the skin. At the cellular level, high PREP levels were seen as a rule in secreting epithelial cells and cells involved in reproduction. Increased PREP expression was seen in most of the tumors studied. PREP expression was higher in malignant than benign tumors, and in ovarian epithelial cancers, there was a trend for increased PREP staining with increased malignancy grade. Results suggest that PREP may be associated with secretory processes as well as in reproduction. A more abundant expression of PREP in malignant than benign tumors suggests that PREP may be associated with expansion and metastasis of tumors. (J Histochem Cytochem 60:706-715, 2012)