Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates
Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates
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DOI：
10.1093/emboj/19.6.1252
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发表时间：
2000-03-15
期刊：
影响因子：
11.4
通讯作者：
González, C
中科院分区：
文献类型：
--
作者：
Lange, BMH;Bachi, A;González, C
为了确定高级真核生物中心体的分子组成，我们对免疫分离的果蝇中心体高富集制剂中的多肽进行了系统的纳米电喷雾串联或MALDI质谱分析。其中鉴定出的一种蛋白是Hsp83，它是高度保守的Hsp90家族的一员，包括已知维持许多蛋白质活性的伴侣蛋白，但被怀疑具有其他基本的、未确定的功能。我们发现，在果蝇和脊椎动物的整个细胞周期的不同发育阶段，总Hsp90库的一小部分定位于中心体。Hsp90与中心体之间的这种关联可以在纯化的中心体和用微管解聚药物治疗后观察到，这两个标准通常用于定义核心中心体成分。果蝇基因突变或用热休克蛋白90抑制剂格尔达霉素处理哺乳动物细胞破坏热休克蛋白90功能，会导致中心体分离和成熟异常、纺锤体异常和染色体分离受损。这表明Hsp90的另一个作用可能是确保中心体的正常功能。
To determine the molecular composition of the centrosome of a higher eukaryote, we carried out a systematic nano-electrospray tandem or MALDI mass spectrometry analysis of the polypeptides present in highly enriched preparations of immunoisolated Drosophila centrosomes. One of the proteins identified is Hsp83, a member of the highly conserved Hsp90 family including chaperones known to maintain the activity of many proteins but suspected to have other essential, unidentified functions. We have found that a fraction of the total Hsp90 pool is localized at the centrosome throughout the cell cycle at different stages of development in Drosophila and vertebrates. This association between Hsp90 and the centrosome can be observed in purified centrosomes and after treatment with microtubule depolymerizing drugs, two criteria normally used to define core centrosomal components. Disruption of Hsp90 function by mutations in the Drosophila gene or treatment of mammalian cells with the Hsp90 inhibitor geldanamycin, results in abnormal centrosome separation and maturation, aberrant spindles and impaired chromosome segregation. This suggests that another role of Hsp90 might be to ensure proper centrosome function.