Effect of epidermal growth factor on the cellular proliferation and phenotype of a neoplastic human salivary intercalated duct cell line or its derivatives.
Effect of epidermal growth factor on the cellular proliferation and phenotype of a neoplastic human salivary intercalated duct cell line or its derivatives.
复制标题
表皮生长因子对肿瘤性人唾液闰管细胞系或其衍生物的细胞增殖和表型的影响。
作者：
W. Aladib;H. Yoshida;M. Sato
在表皮生长因子（EGF）存在下培养肿瘤性人唾液腺闰管细胞系（HSG）及其衍生物，具有肌上皮细胞表型的HSG（HSG-AZA 1）和具有腺泡细胞表型的HSG（HSG-AZA 3），其由5-氮胞苷处理HSG细胞诱导。发生形态学变化;在所有处理的细胞中出现纺锤形或星状细胞，并具有长的胞质突起。主要的改变，如神经元特异性烯醇化酶，神经丝，或突触素的表达，以及形成神经突样结构密集的微纤维和微管，观察到这些细胞与神经元样细胞的表型相似。EGF处理后HSG细胞的贴壁依赖性生长和贴壁非依赖性生长以及HSG-AZA 1细胞的贴壁依赖性生长均受到抑制，而HSG-AZA 3细胞的贴壁依赖性生长则随着EGF浓度的增加而增强。此外，通过统计分析发现，随着EGF添加浓度的增加，培养的HSG细胞的生长受到抑制，培养的HSG-AZA 3细胞的生长受到刺激，而培养的HSG-AZA 1细胞的生长几乎不受EGF处理的影响。HSG、HSG-AZA 1和HSG-AZA 3细胞分别具有1.8 × 10（5）、1.83 × 10（5）和1.34 × 10（5）EGF受体。EGF在HSG-AZA 3细胞中的内化量大于HSG或HSG-AZA 1细胞。这些发现表明，HSG、HSG-AZA 1和HSG-AZA 3细胞转化为神经元样细胞发生在含有EGF的生长培养基中，伴随着以不同方式检测的细胞生长潜能的调节。
A neoplastic human salivary intercalated duct cell line (HSG) and its derivatives, HSG with a myoepithelial cell phenotype (HSG-AZA1) and HSG with an acinar cell phenotype (HSG-AZA3), which were induced by 5-azacytidine treatment of HSG cells, were cultivated in the presence of epidermal growth factor (EGF). Morphological changes occurred; cells that were spindle shaped or stellate and had long cytoplasmic processes appeared in all of the treated cells. Major alterations, such as expression of neuron-specific enolase, neurofilaments, or synaptophysin as well as formation of neurite-like structures densely packed with microfibrils and microtubules, were observed in these cells with a phenotype similar to that of neuron-like cells. Both the anchorage-independent and anchorage-dependent growths of the treated HSG cells and only anchorage-dependent growth of the treated HSG-AZA1 cells were suppressed, whereas the anchorage-dependent growth of the treated HSG-AZA3 cells was enhanced according to the increasing concentrations of EGF. In addition, it has been found by statistical analysis that the growth of HSG cells in culture is suppressed and that of HSG-AZA3 cells in culture is stimulated according to the increasing concentrations of EGF added, whereas the growth of cultured HSG-AZA1 cells is hardly affected by EGF treatment. The HSG, HSG-AZA1, and HSG-AZA3 cell had 1.8 x 10(5), 1.83 x 10(5), and 1.34 x 10(5) EGF receptors, respectively. The amounts of the EGF internalized into cells were larger in HSG-AZA3 cells than in HSG or HSG-AZA1 cells. These findings indicate that the conversion of HSG, HSG-AZA1, and HSG-AZA3 cells into neuron-like cells occurs in growth medium containing EGF, with a concomitant modulation of the growth potentials of the cells which were examined in a different manner.