GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system
GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system
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DOI：
10.1007/s004410050901
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发表时间：
1997-10-01
影响因子：
3.6
通讯作者：
Schneuwly, S
中科院分区：
文献类型：
--
作者：
Ito, K;Sass, H;Schneuwly, S
为了提高果蝇增强器诱捕和转基因菌株中gal4表达细胞的细胞质标记质量，研究了一种新的gal4应答报告基因UAS-tau，其特征为酵母上游激活序列（UAS）控制下的牛tau cDNA。Tau是一种微管相关蛋白，活跃而均匀地分布在所有细胞过程中。单克隆抗牛Tau抗体显示标记细胞的轴突结构，其细节与高尔基浸渍相似。我们证明了UAS-tau系统对于研究胚胎后发育过程中已识别神经元的分化和重组过程特别有用。
To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.