Genetic analysis of temperature-sensitive mutants of HSV-1: the combined use of complementation and physical mapping for cistron assignment.
Genetic analysis of temperature-sensitive mutants of HSV-1: the combined use of complementation and physical mapping for cistron assignment.
复制标题
HSV-1 温度敏感突变体的遗传分析：结合使用互补和物理作图进行顺反子分配。
DOI：
10.1016/0042-6822(83)90084-3
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Schaffer,PA
中科院分区：
文献类型：
--
作者：
Weller,SK;Aschman,DP;Sacks,WR;Coen,DM;Schaffer,PA
到目前为止，代表33个公认的HSV-1互补组中的19个突变体的突变已经被绘制出来。本文报道了HSV-1株Kos株10个突变体的物理图谱位置，它们代表19个互补组中的8个。三个突变体的突变被发现位于坐标0.086和0.194之间--其中两个被精细地映射到坐标0.095和0.108之间--以及七个突变体，介于0.301和0.448之间。在10个突变体Q26中，有1个突变被精细地定位到TK基因3‘端(0.301-0.304)左侧500到1000bpb之间的序列。物理作图数据的可获得性(1)证实了互补试验作为鉴定病毒基因功能的一种手段的有效性，(2)促进了突变体快速分配到新的和识别的顺反子，以及(3)促使对2个互补组中以前不明确的突变体的互补进行了重新评估。因此，本研究中定位的10个突变体先前被分配到8个互补组，假设互补性指数等于或大于2表示2个突变体位于不同的基因中。然而，结合物理作图数据，互补检验的结果现在表明，指数在2到10之间可能反映了基因间或基因内的互补。因此，这10个突变体现在被分配到7个互补组。尽管物理作图数据证实了所分析的8个群体中6个群体以前的互补测试结果，但根据物理作图数据重新评估互补数据已导致对病毒DNA聚合酶基因座和位于UL左侧部分的基因座(由互补组1-10中的突变所代表)的更准确的遗传定义。
To date, mutations in mutants representing 19 of the 33 recognized HSV-1 complementation groups have been mapped. The physical map locations of mutations in 10tsmutants of HSV-1 strain KOS representing 8 of the 19 complementation groups are reported herein. The mutations in three mutants were found to lie between coordinates 0.086 and 0.194—two of these were mapped finely to between coordinates 0.095 and 0.108—and in seven mutants, between 0.301 and 0.448. The mutation in 1 of the 10 mutantstsQ26, was mapped finely to a sequence between 500 and 1000 base pairs to the left of the 3′ end of the TK gene (0.301–0.304). The availability of physical mapping data has (1) confirmed the usefulness of the complementation test as a means of identifying viral gene functions, (2) facilitated the rapid assignment of mutants to new and recognized cistrons, and (3) prompted a reevaluation of previously ambiguous complementation for mutants in 2 complementation groups. Thus, the 10 mutants whosetsmutations were mapped in this study had been assigned previously to 8 complementation groups based on the assumption that complementation indices of 2 or greater signified that 2 mutants were in different genes. Combined with physical mapping data, however, the results of complementation tests now indicate that indices between 2 and 10 may reflect eitherinter- orintragenic complementation. Thus, the 10 mutants have now been assigned to 7 complementation groups. Although physical mapping data have confirmed the results of previous complementation tests for 6 of 8 groups analyzed, reevaluation of complementation data in the light of physical mapping data has resulted in a more precise genetic definition of the locus for viral DNA polymerase and of a locus (represented by mutants in complementation group 1–10) which maps in the left hand portion of UL.