Sensory Innervation of the Calvarial Bones of the Mouse
Sensory Innervation of the Calvarial Bones of the Mouse
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DOI：
10.1002/cne.22049
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发表时间：
2009-07-20
影响因子：
2.5
通讯作者：
Burstein, Rami
中科院分区：
文献类型：
--
作者：
Kosaras, Bela;Jakubowski, Moshe;Burstein, Rami
偏头痛患者经常证实，他们的头痛感觉好像颅骨变形、压碎或断裂（Jakubowski et al. [2006] Pain 125:286-295）。这使我们推测颅骨是由感觉纤维提供的。我们通过外周蛋白（薄髓鞘和无髓鞘纤维的标记物）或降钙素基因相关肽（CGRP；无髓鞘神经纤维更典型的标记物）的免疫染色，研究了出生后和成年小鼠全头标本的冠状和水平切片中颅骨的感觉神经支配。在幼犬中，我们观察到神经束在帽状腱膜和骨膜之间、骨膜和骨骼之间以及骨骼和脑膜之间走行。以及在偶极体内部以不同方向延伸的纤维。一些硬脑膜纤维向大脑前部的软脑膜发出侧支。在成人颅骨中，在缝合处发现了最高浓度的外周蛋白和 CGRP 标记的纤维，它们似乎从硬脑膜中出现。在导道、骨髓和骨膜中也观察到了标记的纤维。与幼犬的情况相反，在成年颅骨的板极中没有发现标记纤维。通过颅骨缝渗入骨膜的脑膜神经可能被定位以介导由颅外组织的病理生理学触发的偏头痛，例如肌肉压痛和颅骨的轻度创伤。鉴于感觉纤维在缝合线中的集中，避免在进行各种神经外科手术的开颅手术的患者中对缝合线钻孔可能是有用的。 J.Comp。内罗尔。 515:331-348, 2009。(c) 2009 Wiley-Liss, Inc.
Migraine sufferers frequently testify that their headache feels as if the calvarial bones are deformed, crushed, or broken (Jakubowski et al. [2006] Pain 125:286-295). This has lead us to postulate that the calvarial bones are supplied by sensory fibers. We studied sensory innervation of the calvaria in coronal and horizontal sections of whole-head preparations of postnatal and adult mice, via immunostaining of peripherin (a marker of thinly myelinated and unmyelinated fibers) or calcitonin gene-related peptide (CGRP; a marker more typical of unmyelinated nerve fibers). In pups, we observed nerve bundles coursing between the galea aponeurotica and the periosteum, between the periosteum and the bone, and between the bone and the meninges; as well as fibers that run inside the diploe in different orientations. Some dural fibers issued collateral branches to the pia at the frontal part of the brain. In the adult calvaria, the highest concentration of peripherin- and CGRP-labeled fibers was found in sutures, where they appeared to emerge from the dura. Labeled fibers were also observed in emissary canals, bone marrow, and periosteum. In contrast to the case in pups, no labeled fibers were found in the diploe of the adult calvaria. Meningeal nerves that infiltrate the periosteum through the calvarial sutures may be positioned to mediate migraine headache triggered by pathophysiology of extracranial tissues, such as muscle tenderness and mild trauma to the skull. In view of the concentration of sensory fibers in the sutures, it may be useful to avoid drilling the sutures in patients undergoing craniotomies for a variety of neurosurgical procedures. J. Comp. Neurol. 515:331-348, 2009. (c) 2009 Wiley-Liss, Inc.