Ton-dependent colicins and microcins: modular design and evolution
Ton-dependent colicins and microcins: modular design and evolution
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DOI：
10.1016/s0300-9084(02)01427-x
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发表时间：
2002-05-01
期刊：
影响因子：
3.9
通讯作者：
Hantke, K
中科院分区：
文献类型：
--
作者：
Braun, V;Patzer, SI;Hantke, K
依赖于质子的大肠杆菌素和微球菌素被敏感细胞主动摄取，消耗细胞质膜质子动力提供的能量。由蛋白TonB、ExbB和ExbD组成的Ton系统是大肠杆菌素和小杆菌素输入所必需的。大肠杆菌素以及外膜转运蛋白含有近端的N-末端的短序列，称为TonB盒，其与TonB相互作用，并且在该点突变损害摄取。在微容器中未发现TonB盒。大肠杆菌素是由功能模块组成的，在进化过程中，这些功能模块已经互换，从而产生新的大肠杆菌素。这些模块定义了与外膜转运基因、TonB、免疫蛋白和活性区域相互作用的位点。具有不同一级结构的六种TonB依赖性微菌素由高度同源的蛋白质加工和输出。其中三种微菌素以未知的方式修饰，它们对儿茶酚铁载体受体具有共同的特异性。(C)2002法国生物化学与生物分子学会/科学与医学版Elsevier SAS。All rights reserved.
Ton-dependent colicins and microcins are actively taken up into sensitive cells at the expense of energy which is provided by the proton motive force of the cytoplasmic membrane. The Ton system consisting of the proteins TonB, ExbB and ExbD is required for colicin and microcin import. Colicins as well as the outer membrane transport proteins contain proximal to the N-terminus a short sequence, called TonB box, which interacts with TonB and in which point mutants impair uptake. No TonB box is found in microcins. Colicins are composed of functional modules which during evolution have been interchanged resulting in new colicins. The modules define sites of interaction with the outer membrane transport genes, TonB, the immunity proteins, and the activity regions. Six TonB-dependent microcins with different primary structures are processed and exported by highly homologous proteins. Three of these microcins are modified in an unknown way and they have in common specificity for catecholate siderophore receptors. (C) 2002 Societe francaise de biochimie et biologic moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.