Rare earth oxides as nanoadditives in 3-D nanocomposite scaffolds for bone regeneration
Rare earth oxides as nanoadditives in 3-D nanocomposite scaffolds for bone regeneration
复制标题
DOI：
10.1039/c0jm01072c
复制
发表时间：
2010-01-01
影响因子：
--
通讯作者：
Seal, Sudipta
中科院分区：
文献类型：
--
作者：
Karakoti, Ajay S.;Tsigkou, Olga;Seal, Sudipta
与不含纳米陶瓷的生物活性玻璃支架相比，含有定制稀土氧化物（即纳米陶瓷）添加剂的生物活性玻璃泡沫纳米复合材料支架被证明可以增强 HMSC（人类间充质干细胞）产生胶原蛋白。不需要添加成骨补充剂来实现这一点。两种不同的纳米二氧化钛制剂成功地融入到3D生物活性玻璃泡沫支架中，并与不含纳米二氧化钛的生物活性支架进行了比较。纳米粒子的单个粒径为 3-5 nm，而团聚体尺寸为 5-15 nm。初步研究表明纳米陶瓷对细胞无毒。培养 10 天后，即使在没有任何成骨补充剂（即抗坏血酸、地塞米松和 β-甘油磷酸盐）的情况下，与不含纳米陶瓷的支架相比，含有纳米陶瓷的支架也显示出增强的 HMSC 成骨细胞分化和胶原蛋白生成。这可能是由于纳米陶瓷的掺入，纳米陶瓷充当氧缓冲剂，从而调节 HMSC 的分化。目前正在进行进一步研究以确定纳米陶瓷控制的氧缓冲对 HMSC 分化的作用。
Nanocomposite scaffolds of bioactive glass foams containing tailor made rare earth oxide (i.e. nanoceria) additives were demonstrated to enhance the production of collagen by HMSCs (Human Mesenchymal Stem Cells) compared to bioactive glass scaffolds without nanoceria. The addition of osteogenic supplements was not required for this to occur. Two different preparations of nanoceria were successfully incorporated in 3-D bioactive glass foam scaffolds and were compared with bioactive scaffolds without nanoceria. The nanoparticles had individual particle sizes of 3-5 nm while the agglomerate size varied from 5-15 nm. Preliminary investigations show that nanoceria is non toxic to the cells. After 10 days of culture, nanoceria containing scaffolds showed enhanced osteoblastic differentiation of HMSCs and collagen production compared to the scaffolds without nanoceria even in the absence of any osteogenic supplements (i.e. ascorbic acid, dexamethasone and beta-glycerophosphate). This could be due to the incorporation of nanoceria, which acts as an oxygen buffer thereby regulating the differentiation of HMSCs. Further investigations are currently underway to determine the role of the nanoceria controlled oxygen buffering on the HMSC differentiation.