Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in Arabidopsis
Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in Arabidopsis
复制标题
KIX-PPD-MYC 阻遏物复合物对 GIF1 的转录抑制控制拟南芥种子大小
DOI：
10.1038/s41467-020-15603-3
复制
发表时间：
2020-04-15
影响因子：
16.6
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Liu, Zupei;Li, Na;Li, Yunhai
种子大小是一个关键的农艺性状，很大程度上决定了植物产量。阐明种子大小调节的分子机制也是发育生物学中的一个重要问题。在这里，我们表明 KIX-PPD-MYC-GIF1 途径在拟南芥种子大小控制中发挥着至关重要的作用。 KIX8/9 和 PPD1/2 的破坏会由于细胞增殖增加和珠被细胞伸长而导致种子变大。在拟南芥中，KIX8/9 和 PPD1/2 与转录因子 MYC3/4 相互作用形成 KIX-PPD-MYC 复合物。 KIX-PPD-MYC 复合物与 GRF 相互作用因子 1 (GIF1) 启动子中的典型 G-box 序列结合，促进种子生长并抑制其表达。遗传分析支持 KIX8/9、PPD1/2、MYC3/4 和 GIF1 在控制种子大小的共同途径中发挥作用。因此，我们的结果揭示了转录因子 MYC3/4 将 KIX8/9 和 PPD1/2 募集到 GIF1 启动子并抑制其表达，从而决定拟南芥种子大小的遗传和分子机制。
Seed size is a key agronomic trait that greatly determines plant yield. Elucidating the molecular mechanism underlying seed size regulation is also an important question in developmental biology. Here, we show that the KIX-PPD-MYC-GIF1 pathway plays a crucial role in seed size control in Arabidopsis thaliana. Disruption of KIX8/9 and PPD1/2 causes large seeds due to increased cell proliferation and cell elongation in the integuments. KIX8/9 and PPD1/2 interact with transcription factors MYC3/4 to form the KIX-PPD-MYC complex in Arabidopsis. The KIX-PPD-MYC complex associates with the typical G-box sequence in the promoter of GRF-INTERACTING FACTOR 1 (GIF1), which promotes seed growth, and represses its expression. Genetic analyses support that KIX8/9, PPD1/2, MYC3/4, and GIF1 function in a common pathway to control seed size. Thus, our results reveal a genetic and molecular mechanism by which the transcription factors MYC3/4 recruit KIX8/9 and PPD1/2 to the promoter of GIF1 and repress its expression, thereby determining seed size in Arabidopsis.