Global protein expression profile response of planktonic Aeromonas hydrophila exposed to chlortetracycline
Global protein expression profile response of planktonic Aeromonas hydrophila exposed to chlortetracycline
复制标题
暴露于金霉素的浮游嗜水气单胞菌的整体蛋白表达谱响应
DOI：
10.1007/s11274-017-2204-y
复制
发表时间：
2017-04-01
影响因子：
4.1
通讯作者：
Lin, Xiangmin
中科院分区：
文献类型：
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作者：
Li, Wanxin;Yao, Zujie;Lin, Xiangmin
嗜水气单胞菌的抗生素耐药现象已成为世界水产养殖业和人类健康面临的严重经济和公共卫生问题。为了探讨嗜水气单胞菌对抗生素的本能适应机制，采用基于iTRAQ(相对和绝对定量等压标签)的定量蛋白质组学技术，比较了金霉素(CTC)胁迫下嗜水气单胞菌在浮游状态下的差异表达，共鉴定出1552个蛋白质，其中285个蛋白质丰度增加，195个丰度降低。生物信息学分析表明，在四氯化碳胁迫下，碳代谢、丙酮酸代谢、糖酵解/糖异生等代谢途径有下调的趋势，而β-内酰胺抗性、核酸降解和氨基酸生物合成过程则更有可能增加。用Q-PCR法进一步从基因水平检测相关的丙酮酸代谢和β-内酰胺耐药过程。因此，了解嗜水气单胞菌对CTC的响应行为将有助于揭示抗生素的适应机制和开发新的抗生素治疗方法。
The antibiotics resistance phenomena of Aeromonas hydrophila has become serious economic and public health problems for the world aquaculture industry and human health care. In this study, to investigate the instinct antibiotics adaptive mechanism of this pathogen, iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) based quantitative proteomics technologies were performed to compare the differential expression of A. hydrophila in planktonic status in response to chlortetracycline (CTC) stress and then identified total 1552 proteins including 285 altered proteins with 90 increasing and 195 decreasing abundance proteins. The following bioinformatics analysis showed that many metabolic metabolism pathways such as carbon metabolism, pyruvate metabolism, and glycolysis/gluconeogenesis were trend to down-regulated whereas β-Lactam resistance, RNA degradation, and amino acids biosynthesis processes were more likely to increase in CTC stress. The related pyruvate metabolism and β-Lactam resistance processes in mRNA level were further measured using the q-PCR method. Thus, an understanding of the behaviors of A. hydrophila in response to CTC would be helpful to reveal the antibiotics adaptive mechanism and for the development of novel antibiotics therapy.