Vaccination of calves with an attenuated cell line of Theileria annulata and the sporozoite antigen SPAG-1 produces a synergistic effect
Vaccination of calves with an attenuated cell line of Theileria annulata and the sporozoite antigen SPAG-1 produces a synergistic effect
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DOI：
10.1016/j.vetpar.2006.06.010
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发表时间：
2006-11-30
影响因子：
2.6
通讯作者：
Hall, Roger
中科院分区：
文献类型：
--
作者：
Darghouth, Mohamed Aziz;Boulter, Nicola R.;Hall, Roger
以往的研究表明，环形泰勒氏菌的子孢子表面抗原SPAG-1和致弱裂殖体感染的突尼斯株CL1分别对同源和异源子孢子攻击具有不同程度的保护作用。我们报告了一项疫苗试验的结果，比较了SPAG-1抗原(作为重组全长His标记蛋白)和减毒细胞系单独或联合使用所诱导的保护水平。在用致死剂量的子孢子虫攻击免疫的牛犊后，结果表明，SPAG-1提供的保护有限(七分之一的牛犊存活)，而减毒的细胞系提供中等保护(七分之三的牛犊恢复)。SPAG-1与减毒细胞株的联合免疫效果最好，所有免疫的牛犊都存活了下来。这些结果，加上一系列寄生虫学和临床参数，证明了在接种热带泰勒虫病疫苗时，通过结合子孢子和裂殖体抗原提供的增强保护。(C)2006爱思唯尔B.V.保留所有权利。
The sporozoite surface antigen, SPAG-1 and the attenuated schizont infected Tunisian line CL1 of Theileria annulata have been shown, in previous studies, to induce variable levels of protection against homologous and heterologous sporozoite challenge, respectively. We report here the result of a vaccination trial comparing the protection level induced by the SPAG-1 antigen (as a recombinant full length His tagged protein) and the attenuated cell line, used singly or in combination. The results, after challenge of immunised calves with a lethal dose of sporozoites, show that SPAG-1 provides limited protection (one out of seven calves surviving), while the attenuated cell line provides moderate protection (three out of seven calves recovered). The combination of SPAG-1 and the attenuated cell line induced the best protection as indicated by the survival of all the vaccinated calves. These results, together with a range of parasitological and clinical parameters, demonstrate the enhanced protection provided by combining sporozoite and schizont antigens in vaccination against tropical theileriosis. (c) 2006 Elsevier B.V. All rights reserved.