Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy
Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy
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DOI：
10.1128/aac.00628-15
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发表时间：
2015-08-01
影响因子：
4.9
通讯作者：
Perilli, Mariagrazia
中科院分区：
文献类型：
--
作者：
Bottoni, Carlo;Marcoccia, Francesca;Perilli, Mariagrazia
在意大利中部城市污水水样中鉴定出两种新的天然CphA金属β-内酰胺酶，CphA 4和CphA 5酶。与CphA相比，CphA 4和CphA 5酶显示出许多点突变。这些酶具有仅集中于碳青霉烯类的狭窄底物谱。与亚胺培南、厄他培南和比阿培南相比，CphA 5的k（cat）值分别比CphA 4高约40、12和97倍。
Two new natural CphA metallo-beta-lactamases, the CphA4 and CphA5 enzymes, were identified in water samples from municipal sewage in central Italy. Compared to CphA, the CphA4 and CphA5 enzymes showed numerous point mutations. These enzymes have a narrow spectrum of substrates focused on carbapenems only. CphA5 showed k(cat) values about 40-, 12-, and 97-fold higher than those observed for CphA4 versus imipenem, ertapenem, and biapenem, respectively.