Side population cells in the human vocal fold
Side population cells in the human vocal fold
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DOI：
10.1177/000348940711601110
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发表时间：
2007-11-01
影响因子：
1.4
通讯作者：
Ito, Juichi
中科院分区：
文献类型：
--
作者：
Yamashita, Masaru;Hirano, Shigeru;Ito, Juichi
目的：声带的再生过程，或折叠中是否存在干细胞，目前尚不清楚。侧群(SP)细胞被定义为具有排除DNA结合染料Hoechst 33342的能力的细胞。它们被认为是一个富含干细胞的细胞群，可以通过荧光激活的细胞分选器从非SP细胞中分离出来。本研究旨在确定人类声带中是否存在SP细胞，作为阐明声带再生机制的第一步。其中2例用于荧光激活的细胞分类分析，5例用于SP细胞表达的三磷酸腺苷结合盒转运体家族成员ABCG2的免疫组织化学分析。ABCG2阳性细胞分布于整个声带的上皮组织和皮下组织。结论：本研究初步证实了人声带中存在SP细胞。需要进一步的研究来阐明这些细胞在声带中是如何工作的，特别是在再生过程中。
Objectives: The regenerative processes of the vocal fold, or the existence of stem cells in the folds, are unknown. Side population (SP) cells are defined as cells that have the ability to exclude the DNA binding dye, Hoechst 33342. They are regarded as a cell population enriched with stem cells and can be isolated from non-SP cells by a fluorescence-activated cell sorter. This study was designed to determine whether SP cells exist in the human vocal fold, as a first step in elucidating the regenerative mechanisms of the vocal fold.Methods: Seven human excised larynges were used in this study. Two were used for fluuorescence- activated cell sorter analysis, and 5 were subjected to immunohistochemical analysis with antibodies against an adenosine triphosphate binding cassette transporter family member, ABCG2, which is expressed in SP cells.Results: The number of SP cells in the human vocal fold was about 0.2% of the total number of cells. ABCG2-positive cells were identified in both the epithelium and subepithelial tissue throughout the entire vocal fold.Conclusions: This preliminary study demonstrated the existence of SP cells in the human vocal fold. Further studies are warranted to clarify how these cells work in the vocal fold, particularly in the regenerative process.