Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of Clostridiales in Crohn's disease involving the ileum
Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of Clostridiales in Crohn's disease involving the ileum
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DOI：
10.1038/ismej.2007.52
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发表时间：
2007-09-01
期刊：
影响因子：
11
通讯作者：
Simpson, Kenneth W.
中科院分区：
文献类型：
--
作者：
Baumgart, Martin;Dogan, Belgin;Simpson, Kenneth W.
肠道细菌越来越多地被认为是克罗恩病发生发展的关键因素，但驱动炎症反应的复杂多菌肠道环境的特定成分尚未解决。本研究探讨回肠黏膜相关菌群在克罗恩病中的作用。采用非培养的细菌多样性分析(16S rDNA文库分析、定量聚合酶链式反应和荧光原位杂交)和培养细菌的分子特征相结合的方法，检测了13例克罗恩病患者(13例)、8例局限于结肠的克罗恩病患者(8例)和7例健康人(7例)的回肠黏膜相关菌群。从回肠粘膜构建的16S rDNA文库分析显示，有9个分支按来源分离(P
Intestinal bacteria are implicated increasingly as a pivotal factor in the development of Crohn's disease, but the specific components of the complex polymicrobial enteric environment driving the inflammatory response are unresolved. This study addresses the role of the ileal mucosa-associated microflora in Crohn's disease. A combination of culture-independent analysis of bacterial diversity (16S rDNA library analysis, quantitative PCR and fluorescence in situ hybridization) and molecular characterization of cultured bacteria was used to examine the ileal mucosa-associated flora of patients with Crohn's disease involving the ileum (13), Crohn's disease restricted to the colon (CCD) (8) and healthy individuals (7). Analysis of 16S rDNA libraries constructed from ileal mucosa yielded nine clades that segregated according to their origin (P