AN ADDITIONAL LIMB CAN BE INDUCED FROM THE FLANK OF THE CHICK-EMBRYO BY FGF4
AN ADDITIONAL LIMB CAN BE INDUCED FROM THE FLANK OF THE CHICK-EMBRYO BY FGF4
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DOI：
10.1006/bbrc.1995.1572
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发表时间：
1995-04-26
影响因子：
3.1
通讯作者：
NOJI, S
中科院分区：
文献类型：
--
作者：
OHUCHI, H;NAKAGAWA, T;NOJI, S
为了阐明是什么启动了肢的形成，我们试图通过感染逆转录病毒或植入细胞从鸡胚的Rank诱导额外的肢。我们在这里报告，一个额外的肢体可以形成从侧翼时，我们植入成纤维细胞生长因子4（FGF4）表达细胞到侧板中胚层在肢芽前阶段。在一个新形成的肢芽，表达的Sonic刺猬和鸡Fgf4，这是真实的形态发生信号的极化活动区和顶端外胚层嵴，分别由植入的细胞诱导。因此，可以得出结论，肢体发育的能力是存在沿着侧翼的鸡胚和异位应用的FGF 4在前肢芽阶段可以改变发育的命运侧翼细胞成为肢体细胞。本实验系统将有助于进一步阐明肢体是如何形成的。(C)出版社：Academic Press
To elucidate what initiates formation of the limb, we have attempted to induce an additional limb from the Rank of the chick embryo by infecting retrovirus or implanting cells. We report here that an additional limb can be formed from the flank when we implant fibroblast growth factor 4 (Fgf4)-expressing cells into the lateral plate mesoderm at the pre-limb bud stage. In a newly formed limb bud, expressions of both Sonic hedgehog and chick Fgf4, which are authentic morphogenetic signals from the zone of polarizing activity and the apical ectodermal ridge, respectively, are induced by the implanted cells. Thus, it is concluded that the competence for limb development is present along the flank of the chick embryo and that FGF4 applied ectopically at the pre-limb bud stage can alter the developmental fate of flank cells to become limb cells. The present experimental system will contribute to a further elucidation on how the limb is formed. (C) 1995 Academic Press, Inc.