MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE
MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE
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DOI：
10.1002/rcm.1290080904
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发表时间：
1994-09-01
影响因子：
2
通讯作者：
SIEMIENIAK, D
中科院分区：
文献类型：
--
作者：
BAI, J;LIU, YH;SIEMIENIAK, D
利用Nafion活性底物中的3-羟基烟酸基质辅助激光解吸/电离，利用飞行时间质谱仪检测限制性内切酶消化的双链质粒DNA。通过最低限度的样品纯化，检测到高达267个碱基对的DNA链，尽管只是对应于单链DNA的物种。
Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single-stranded DNA.