Icariin-conditioned serum engineered with hyaluronic acid promote repair of articular cartilage defects in rabbit knees
Icariin-conditioned serum engineered with hyaluronic acid promote repair of articular cartilage defects in rabbit knees
复制标题
透明质酸淫羊藿苷条件血清促进兔膝关节软骨缺损的修复
DOI：
10.1186/s12906-019-2570-0
复制
发表时间：
2019-07-03
影响因子：
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通讯作者：
Shang, Man
中科院分区：
文献类型：
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作者：
Zhang, Juntao;Zhang, Donglin;Shang, Man
背景骨软骨缺损多由创伤或关节退行性变引起。关节软骨再生能力差是骨软骨缺损的一个棘手问题。现代的治疗策略主要集中在生物活性材料的软骨组织工程。本研究旨在制备淫羊藿苷条件血清（ICS）和透明质酸（HA），并探讨其对兔膝关节骨软骨组织修复的作用。方法将原代软骨细胞与不同浓度的淫羊藿苷条件血清共同孵育，检测细胞增殖率和糖胺聚糖（GAG）分泌量。分别在兔右膝关节内注射0.5mL生理盐水（NS）、ICS、HA和ICS + HA。ICRS评分用于评估宏观软骨再生。组织学和免疫组织化学分析，包括H&E，番红O，甲苯胺蓝和胶原II染色，用于确定软骨修复和软骨细胞再生的。Results0.94 g/kg的低剂量淫羊藿苷被确定为具有显着促进软骨细胞增殖和增加GAG的分泌。ICS与HA联合治疗的兔股骨髁与自体软骨融合，软骨下骨再生更多。ICS与HA联合应用可促进软骨缺损的修复，增加软骨的新生。ConclusionsThese结果表明ICS与HA联合应用可促进软骨缺损的修复反应，并可能应用于基于生物活性材料的软骨再生治疗。
BackgroundOsteochondral defects mostly occur as a result of trauma or articular degeneration. The poor regenerative ability of articular cartilage remains osteochondral defects are a tricky problem to deal with. The modern treatment strategies mainly focus on cartilage tissue engineering with bioactive materials. In this study, we aimed to develop icariin conditioned serum (ICS) together with hyaluronic acid (HA) and determine their ability in reparing osteochondral tissue in a critical-sized defect in rabbit knees.MethodsPrimary chondrocytes were incubated with serum conditioned with icariin at different concentrations, then cell proliferation rates and glycosaminoglycan (GAG) secretion were detected. Rabbits were treated with intra-articular injection of 0.5 mL normal saline (NS), ICS, HA and ICS + HA in the right knee joint, respectively. ICRS scores were used to assess the macroscopic cartilage regeneration. Histological and immunohistochemical analysis including H&E, Safranin O, toluidine blue and collagen II staining were used to determine the repair of cartilage and the regeneration of chondrocytes.ResultsIcariin at a low dose of 0.94 g/kg was identified to have significantly promoted the proliferation of chondrocytes and enhance the secretion of GAG. Femoral condyle from rabbits treated by ICS together with HA was observed to be integrated with native cartilage and more subchondral bone regeneration. ICS together with HA could promote repair of the cartilage defect and increase the neoformation of cartilage.ConclusionsThese results demonstrated the potential of ICS combined with HA to promote reparative response in cartilage defects and the possible application in bioactive material based cartilage regeneration therapies.