Patterns of colocalization of neuronal nitric oxide synthase and somatostatin-like immunoreactivity in the mouse hippocampus: Quantitative analysis with optical disector
Patterns of colocalization of neuronal nitric oxide synthase and somatostatin-like immunoreactivity in the mouse hippocampus: Quantitative analysis with optical disector
复制标题
DOI：
10.1016/j.neuroscience.2004.01.027
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Kosaka, T
中科院分区：
文献类型：
--
作者：
Jinno, S;Kosaka, T
在一些脑区，以前的研究报道神经元型一氧化氮合酶(NNOS)和生长抑素(SOM)经常共存。在海马区，nNOS和SOM主要在GABA能非主神经元表达。在这里，我们使用光学偏转器估计了nNOS和SOM在小鼠海马区的免疫细胞化学共存。在杏仁角和齿状回，我们仅见到少数nNOS免疫反应(IR)/SOM样免疫反应(LIR)神经元。它们主要分布在亚扪角的东方层和齿状门处。NNOS-IR/SOM-LIR神经元通常具有特征性的大胞体和粗大树突，而nNOS-IR/SOM阴性神经元大多表现为小胞体和稀疏树突。定量数据显示，双标记细胞仅占整个海马区nNOS-IR和SOM-LIR神经元的4%和7%。我们还发现nNOS和SOM在共定位程度上存在板层和背腹的差异。在CA1腹侧层(24%)、CA3背侧透明层(29%)和齿状门背侧(32%)，含SOM免疫反应阳性神经元的比例较高，其余各层较低。另一方面，在CA3背侧透明层(19%)和齿状背侧门区(28%)，含有nNOS免疫反应的SOM-LIR神经元的比例较高，而在其他各层中则很低。NNOS、SOM和谷氨酸脱羧酶轴突终末的免疫荧光三重标记表明，部分nNOS-IR/SOM-LIR神经元可能是树突状抑制细胞。本研究结果表明，nNOS和SOM在小鼠海马区罕见地共存，提示双标记细胞可能是海马区GABA能非主神经元的一个特殊亚群。(C)2004年IBRO。爱思唯尔有限公司出版。保留所有权利。
In some brain regions, previous studies reported the frequent coexistence between neuronal nitric oxide synthase (nNOS) and somatostatin (SOM). In the hippocampus, nNOS and SOM were mainly expressed in GABAergic nonprincipal neurons. Here we estimated the immunocytochemical colocalization of nNOS and SOM in the mouse hippocampus using the optical disector. Both in the Ammon's horn and dentate gyrus, we encountered only a few nNOS-immunoreactive (IR)/SOM-like immunoreactive (LIR) neurons. They were mainly located in the stratum oriens of the Ammon's horn and in the dentate hilus. The nNOS-IR/SOM-LIR neurons usually showed characteristic large somata with thick dendrites, whereas the majority of nNOS-IR/SOM-negative neurons showed small somata with thin dendrites. Quantitative data revealed that the double-labeled cells represented only 4% and 7% of nNOS-IR neurons and SOM-LIR neurons, respectively, in the whole area of the hippocampus. We also found the laminar and dorsoventral differences in the degree of colocalization between nNOS and SOM. The percentages of nNOS-IR neurons containing SOM-like immunoreactivity were relatively high in the stratum oriens of the ventral CA1 region (24%), stratum lucidum of the dorsal CA3 region (29%) and dorsal dentate hilus (32%), but they were quite low in the other layers. On the other hand, the percentages of SOM-LIR neurons containing nNOS immunoreactivity were somewhat high in the stratum lucidum of the dorsal CA3 region (19%) and dorsal dentate hilus (28%), whereas they were very low in the other layers. Immunofluorescent triple labeling of axon terminals for nNOS, SOM and glutamic acid decarboxylase indicated that some nNOS-IR/SOM-LIR neurons might be dendritic inhibitory cells. The present results show the infrequent colocalization of nNOS and SOM in the mouse hippocampus, and also suggest that the double-labeled cells may be a particular subpopulation of hippocampal GABAergic nonprincipal neurons. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.