Peripheral CD4+/CD8+T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals
Peripheral CD4+/CD8+T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals
复制标题
DOI：
10.1016/s1386-6532(00)00080-9
复制
发表时间：
2000-08-01
影响因子：
8.8
通讯作者：
Sridharan, G
中科院分区：
文献类型：
--
作者：
Kannangai, R;Prakash, KJ;Sridharan, G
背景：流式细胞术是估计CD4/CD8计数的标准方法；但该仪器的高初始投资和昂贵的试剂使其对像印度这样的发展中国家的大多数中心来说负担不起。目的：评估替代系统用于估计正常南印度成人的CD4和CD8计数的可行性，并验证该方法在监测HIV血清阳性个体的计数的有效性。研究设计：46名正常健康成人和68名HIV血清阳性者参加了这项横断面研究，他们都属于南印度语系。HIV血清阳性者包括54例HIV-1、9例HIV-2和5例HIV 1&2感染者，其中一种商业免疫印迹试剂盒进行了血清学确认。使用Capcellia CD4：CD8全血检测，这是一种基于免疫捕获ELISA法的试剂盒，来自法国Sanof Diagnostics Pasteur，在程序中进行了一些修改，以测量CD4和CD8计数。结果：正常人、无症状HIV感染者和有症状HIV感染者外周血中CD4细胞计数分别为1048、746和424，CD8细胞计数分别为595、889和732。在艾滋病毒血清阴性的健康成人和无症状(P<；0.001)以及无症状和有症状的艾滋病毒感染者(P<；005)之间，观察到CD_4细胞计数有显著差异。无症状HIV-2感染者的平均CD4计数显著高于无症状HIV-1感染者(P<；0.05)。结论：这是一种用户友好的检测方法，可以替代流式细胞术来评估发展中国家的外周T淋巴细胞亚群。该检测系统对酶联免疫吸附试验技术具有一定的局限性。(C)2000 Elsevier Science B.V.保留所有权利。
Background: Flow cytometry is the standard method for the estimation of CD4/CD8 counts; but the high initial investment for this instrument and costly reagents make it unaffordable to most of the centers in a developing country like India. Objectives: To evaluate the feasibility of an alternate system for the estimation of CD4 and CD8 counts in normal south Indian adults and validate the usefulness of this assay to monitor the counts in HIV seropositive individuals. Study design: Forty-six normal healthy adults and 68 HIV seropositive individuals both belonging to south Indian linguistic groups were enrolled in this cross-sectional study. The HIV seropositive individuals included 54 HIV-1, 9 HIV-2 and 5 HIV 1&2 infected individuals serologically confirmed by one of the commercial Immunoblot kits. The Capcellia CD4:CD8 whole blood assay, an immune-capture ELISA based kit from Sanof DIAGNOSTICS Pasteur, (France) was used with a few modifications in the procedure to measure the CD4 and CD8 counts. Results: The mean CD4 cell counts were 1048 (central 95 centile only), 746 and 424 for the normal healthy adults, asymptomatic HIV seropositives and symptomatic HIV patients, respectively, and the mean CD8 counts were 595, 889 and 732, respectively. Statistically significant differences were observed in the CD4 cell counts between HIV seronegative healthy adults and asymptomatic (P < 0.001) as well as asymptomatic and symptomatic (P < 0.05) HIV infected individuals. The mean CD4 counts of asymptomatic HIV-2 infected individuals was significantly higher than the counts of asymptomatic HIV-1 infected individuals (P < 0.05). Conclusions: This is an user friendly test and can be an alternate to flow cytometry for the estimation of peripheral T-lymphocyte subsets in developing countries. The assay system has certain limitations inherent to ELISA techniques. (C) 2000 Elsevier Science B.V. Ail rights reserved.