Peritubular dentin formation: Crystal organization and the macromolecular constituents in human teeth
Peritubular dentin formation: Crystal organization and the macromolecular constituents in human teeth
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DOI：
10.1006/jsbi.1999.4096
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发表时间：
1999-06-01
影响因子：
3
通讯作者：
Siddiqui, F
中科院分区：
文献类型：
--
作者：
Weiner, S;Veis, A;Siddiqui, F
小管周围牙本质（PTD）是一种相对致密的矿化组织，围绕着冠状牙本质的小管。它主要由碳酸盐磷灰石晶体和少量胶原蛋白组成。通过研究从粉末制剂中分离出的相对致密的颗粒，对其形成方式进行了研究。对PTD颗粒的电镜检查，包括三维图像重建和电子衍射，表明PTD晶体的组织与邻近的管间牙本质（ITD）非常相似。后者含有较多的胶原蛋白，碳酸盐磷灰石晶体与胶原基质密切相关。存在于PTD颗粒中的蛋白质在脱钙后是可溶的，并用污渍全部染色。与管间牙本质的磷酸化蛋白相比，主蛋白具有更高的分子量和完全不同的氨基酸组成。过渡段与过渡段之间的界面具有结构连续性。这些数据显示了两种不同的碳酸盐磷灰石矿化组织是如何利用不同的基质蛋白在同一器官内连续组织和形成的。(C) 1999学术出版社。
Peritubular dentin (PTD) is a relatively dense mineralized tissue that surrounds the tubules of coronal tooth dentin. It is composed mainly of crystals of carbonated apatite together with a small amount of collagen. Its mode of formation has been investigated by studying the relatively dense particles isolated from a powdered preparation. Electron microscopic examination of the PTD particles, including 3-dimensional image reconstruction and electron diffraction, shows that the organization of the crystals of PTD is very similar to that of the adjacent intertubular dentin (ITD). The latter contains relatively large amounts of collagen and the carbonated apatite crystals are closely associated with the collagen matrix. The proteins present in the PTD particles are soluble after decalcification and stain with Stains All. The principal protein has higher molecular weight and a quite different amino acid composition than the phosphophoryns of the intertubular dentin. The interface between the PTD and the ITD shows structural continuity. These data show how two distinct carbonated apatite-based mineralized tissues can be organized and formed contiguously within the same organ by utilizing different sets of matrix proteins. (C) 1999 Academic Press.