Metal ions may suppress or enhance cellular differentiation in Candida albicans and Candida tropicalis biofilms
Metal ions may suppress or enhance cellular differentiation in Candida albicans and Candida tropicalis biofilms
复制标题
DOI：
10.1128/aem.02711-06
复制
发表时间：
2007-08-01
影响因子：
4.4
通讯作者：
Turner, Raymond J.
中科院分区：
文献类型：
--
作者：
Harrison, Joe J.;Ceri, Howard;Turner, Raymond J.
白色念珠菌和热带念珠菌是多态性真菌，其产生以多种细胞形态型为特征的抗微生物剂抗性生物膜群落。本研究探讨了细胞类型的相互转换和药物和金属的阻力，以及社区组织在这些微生物被暴露于金属离子的生物膜。为了研究这一点，念珠菌生物膜生长在含有金属离子梯度阵列的微量滴定板中或在卡尔加里生物膜装置中进行高通量敏感性测试。通过活细胞计数、四唑盐还原、光学显微镜和激光共聚焦扫描显微镜结合三维可视化来评价生物膜形成和抗真菌药耐药性。我们发现，亚抑制浓度的某些金属离子（CrO 42-，Co 2+，Cu 2+，Ag+，Zn 2+，Cd 2+，He 2+，Pb 2+，AsO 2-，和SeO 32-）引起生物膜结构的变化，通过阻断或引发酵母和菌丝细胞类型之间的过渡。从这些数据中辨别出四种不同的生物膜群落结构类型，它们被指定为“圆顶”、“层饼”、“扁平”和“菌丝体”。“这项研究表明，念珠菌生物膜种群可能会对金属离子产生反应，形成具有不同细胞分化模式的细胞-细胞和固体表面附着组合。
Candida albicans and Candida tropicalis are polymorphic fungi that develop antimicrobial-resistant biofilm communities that are characterized by multiple cell morphotypes. This study investigated cell type interconversion and drug and metal resistance as well as community organization in biofilms of these microorganisms that were exposed to metal ions. To study this, Candida biofilms were grown either in microtiter plates containing gradient arrays of metal ions or in the Calgary Biofilm Device for high-throughput susceptibility testing. Biofilm formation and antifungal resistance were evaluated by viable cell counts, tetrazolium salt reduction, light microscopy, and confocal laser scanning microscopy in conjunction with three-dimensional visualization. We discovered that subinhibitory concentrations of certain metal ions (CrO42-, Co2+, Cu2+, Ag+, Zn2+, Cd2+, He2+, Pb2+, AsO2-, and SeO32-) caused changes in biofilm structure by blocking or eliciting the transition between yeast and hyphal cell types. Four distinct biofilm community structure types were discerned from these data, which were designated "domed," "layer cake," "flat," and "mycelial." This study suggests that Candida biofilm populations may respond to metal ions to form cell-cell and solid-surface-attached assemblages with distinct patterns of cellular differentiation.