Different response of photosystem II to short and long-term drought stress in Arabidopsis thaliana
Different response of photosystem II to short and long-term drought stress in Arabidopsis thaliana
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拟南芥光系统II对短期和长期干旱胁迫的不同响应
DOI：
10.1111/ppl.12438
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发表时间：
2016-10-01
影响因子：
6.4
通讯作者：
Yuan, Shu
中科院分区：
文献类型：
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作者：
Chen, Yang-Er;Liu, Wen-Juan;Yuan, Shu
以拟南芥为材料，研究了短期和长期干旱胁迫对光系统II（PSII）和氧化胁迫的影响。在干旱胁迫下，叶绿素（Chl）含量、Chl荧光、相对含水量和放氧能力逐渐下降，类囊体结构逐渐受到破坏。短期干旱胁迫导致捕光复合物II（LHCII）的快速分解。而PSII二聚体在短期干旱胁迫下基本保持稳定，仅在干旱胁迫15 d后才显著下降。类囊体膜蛋白的免疫印迹分析表明，大多数光系统蛋白在胁迫后下降，特别是Lhcb 5，Lhcb 6和PsbQ蛋白。然而，令人惊讶的是，PsbS显着增加后，长期干旱胁迫，这是与胁迫后大幅增加的非光化学猝灭（NPQ）。我们的研究结果表明，PSII-LHCII超复合物和LHCII组装体在防止干旱胁迫下的光损伤PSII发挥重要作用。
Short- and long-term drought stress on photosystem II (PSII) and oxidative stress were studied in Arabidopsis thaliana. Under drought stress, chlorophyll (Chl) content, Chl fluorescence, relative water content and oxygen evolution capacity gradually decreased, and the thylakoid structure was gradually damaged. Short-term drought stress caused a rapid disassembly of the light-harvesting complex II (LHCII). However, PSII dimers kept stable under the short-term drought stress and significantly decreased only after 15 days of drought stress. Immunoblotting analysis of the thylakoid membrane proteins showed that most of the photosystem proteins decreased after the stress, especially for Lhcb5, Lhcb6 and PsbQ proteins. However, surprisingly, PsbS significantly increased after the long-term drought stress, which is consistent with the substantially increased non-photochemical quenching (NPQ) after the stress. Our results suggest that the PSII-LHCII supercomplexes and LHCII assemblies play an important role in preventing photo-damages to PSII under drought stress.