Effects of buthionine sulfoximine on cd-binding Peptide levels in suspension-cultured tobacco cells treated with cd, zn, or cu.
Effects of buthionine sulfoximine on cd-binding Peptide levels in suspension-cultured tobacco cells treated with cd, zn, or cu.
复制标题
丁硫氨酸亚砜亚胺对用 cd、zn 或 cu 处理的悬浮培养烟草细胞中 cd 结合肽水平的影响。
DOI：
10.1104/pp.84.3.574
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发表时间：
1987
期刊：
影响因子：
7.4
通讯作者：
George J. Wagner
中科院分区：
文献类型：
--
作者：
R. Neil Reese;George J. Wagner
在暴露于丁硫氨酸亚磺酰亚胺的培养烟草细胞中测量了细胞生长的抑制和镉结合肽的积累。这种谷胱甘肽代谢抑制剂在没有 Cd 的情况下几乎没有或没有引起生长减少（0.1 毫摩尔），但在暴露于丁硫氨酸亚磺酰亚胺和 >/=22 微摩尔 Cd 的培养物中，生长大大减少。细胞生长的减少与镉结合肽水平的降低和游离镉水平的增加直接相关。锌仅在所检测的最高水平（900-1800微摩尔锌）下抑制烟草细胞的生长，但丁硫氨酸亚磺酰亚胺没有额外的显着效果。对于 Cu（45-90 微摩尔）也观察到类似的结果。结果表明，植物镉肽的合成涉及γ-谷氨酰半胱氨酸合成酶或相关酶，并且烟草细胞中锌的积累不会导致重要的镉肽配体的形成。
Inhibition of cell growth and accumulation of Cd-binding peptide were measured in cultured tobacco cells exposed to buthionine sulfoximine. This inhibitor of glutathione metabolism caused little or no reduction of growth (at 0.1 millimolar) in the absence of Cd, but growth was greatly reduced in cultures exposed to buthionine sulfoximine and >/=22 micromolar Cd. Decreased cell growth was directly correlated with decreased levels of Cd-binding peptide and increased levels of what is thought to be free Cd. Zinc inhibited growth of tobacco cells only at the highest levels examined (900-1800 micromolar Zn), but buthionine sulfoximine had no additional significant effect. Similar results were observed for Cu (45-90 micromolar). Results suggest that synthesis of plant Cd-peptide involves gamma-glutamylcysteine synthetase or a related enzyme and that Zn accumulation in tobacco cells does not cause formation of significant Cd-peptide ligand.