Ethylene response factors Pp4ERF24 andPp12ERF96 regulate blue light-induced anthocyanin biosynthesis in‘Red Zaosu’ pear fruits byinteracting withMYB114
Ethylene response factors Pp4ERF24 andPp12ERF96 regulate blue light-induced anthocyanin biosynthesis in‘Red Zaosu’ pear fruits byinteracting withMYB114
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姜花短链醇脱氢酶是异位罗勒烯生物合成的参与者
DOI：
10.1007/s1110
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发表时间：
2018
影响因子：
5.1
通讯作者：
Yuanwen Teng
中科院分区：
文献类型：
--
作者：
Junbei Ni;Songling Bai;Yuan Zhao;Minjie Qian;Ruiyan Tao;Lei Yin;Ling Gao;Yuanwen Teng
通过大规模挖掘天然种质资源中的SNP并结合高分辨率关联作图，鉴定潜在的转录因子（TF）基因衍生的调节荚果和种子产量组成性状的天然SNP等位变异体，对于理解鹰嘴豆数量产量性状的复杂遗传结构至关重要。在这些方面，目前的研究采用了全基因组GBS（测序基因分型）和靶向基因扩增子重测序为基础的同步SNP发现和基因分型测定，发现了1611个新的SNP从736 TF基因物理定位在8条染色体和非锚定支架的鹰嘴豆基因组。这些SNPs的结构和功能注释在不同的同义和非同义编码以及非编码调控和内含子序列组成部分的鹰嘴豆TF基因。将1611个TF基因SNPs的基因分型信息与鹰嘴豆6个主要荚果和种子产量性状的多地点/多年田间表型数据进行关联分析。这基本上鉴定了27个TF基因衍生的SNP，其表现出与鹰嘴豆的6个主要产量性状显著相关，即50%开花天数（DF）、株高（PH）、分支数（BN）、荚数（PN）、种子数（SN）和种子重量（SW）。这些性状相关的SNPs单独和组合分别解释了10-23%和32%的表型变异的研究产量构成性状。有趣的是，在编码SBP的五个潜在候选TF基因中，（鳞状细胞癌启动子结合蛋白），SNF 2（蔗糖非发酵2），GRAS [赤霉酸不敏感（GAI）-GAI（RGA）-SCARECROW（SCR）的阻遏物]，bZIP（碱性亮氨酸拉链）和LOB（侧向器官边界）-结构域蛋白分别与DF、PH、BN、PN、SN和SW性状密切相关，在鹰嘴豆中被发现最有前途。我们描绘的功能相关的分子标记（TF和天然SNP等位基因）有可能通过开发高豆荚和种子产量的鹰嘴豆品种来加速标记辅助遗传增强。
Identification of potential transcription factor (TF) gene-derived natural SNP allelic variants regulating pod and seed yield component traits by large-scale mining and genotyping of SNPs in natural germplasm accessions coupled with high-resolution association mapping is vital for understanding the complex genetic architecture of quantitative yield traits in chickpea. In these perspectives, the current study employed a genome-wide GBS (genotyping-by-sequencing) and targeted gene amplicon resequencing-based simultaneous SNP discovery and genotyping assays, which discovered 1611 novel SNPs from 736 TF genes physically mapped on eight chromosomes and unanchored scaffolds ofkabulichickpea genome. These SNPs were structurally and functionally annotated in diverse synonymous and non-synonymous coding as well as non-coding regulatory and intronic sequence components of chickpea TF genes. A high-resolution genetic association analysis was performed by correlating the genotyping information of 1611 TF gene-based SNPs with multi-location/years field phenotyping data of six major pod and seed yield traits evaluated in a constituted association panel (326desiandkabuligermplasm accessions) of chickpea. This essentially identified 27 TF gene-derived SNPs exhibiting significant association with six major yield traits, namely days to 50% flowering (DF), plant height (PH), branch number (BN), pod number (PN), seed number (SN) and seed weight (SW) in chickpea. These trait-associated SNPs individually and in combination explained 10–23% and 32% phenotypic variation respectively for the studied yield component traits. Interestingly, novel non-synonymous coding SNP allelic variants in five potential candidate TF genes encoding SBP (squamosal promoter binding protein), SNF2 (sucrose non-fermenting 2), GRAS [Gibberellic acid insensitive (GAI)-Repressor of GAI (RGA)-SCARECROW (SCR)], bZIP (basic leucine zipper) and LOB (lateral organ boundaries)-domain proteins associated strongly with DF, PH, BN, PN, SN and SW traits respectively were found most promising in chickpea. The functionally relevant molecular signatures (TFs and natural SNP alleles) delineated by us have potential to accelerate marker-assisted genetic enhancement by developing high pod and seed yielding cultivars of chickpea.