Sequentially Shifted Excitation Raman Spectroscopy: Novel Algorithm and Instrumentation for Fluorescence-Free Raman Spectroscopy in Spectral Space
Sequentially Shifted Excitation Raman Spectroscopy: Novel Algorithm and Instrumentation for Fluorescence-Free Raman Spectroscopy in Spectral Space
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DOI：
10.1366/12-06852
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发表时间：
2013-08-01
影响因子：
3.5
通讯作者：
Wise, Kent L.
中科院分区：
文献类型：
--
作者：
Cooper, John B.;Abdelkader, Mohamed;Wise, Kent L.
一种新颖的手持式拉曼光谱仪。该光谱仪采用温度控制的分布式布拉格反射二极管激光器，使仪器能够在顺序移动激发模式下运行，以消除荧光背景、固定模式噪声和室内灯光，同时将拉曼数据保持在真实的光谱空间中。该仪器采用经济高效的设计，可以快速采集移位激励数据，移位时间损失小于 2 秒。使用一种新颖的算法从平移激发光谱中提取拉曼数据，该算法通常比光谱空间中运行的传统平移激发算法快三个数量级。通过与 FT-拉曼、标准差光谱、位移激发拉曼差异光谱 (SERDS) 和传统多位移激发方法的比较，证明了仪器和算法在背景去除和信噪比方面的优越性。
A novel Raman spectrometer is presented in a handheld format. The spectrometer utilizes a temperature-controlled, distributed Bragg reflector diode laser, which allows the instrument to operate in a sequentially shifted excitation mode to eliminate fluorescence backgrounds, fixed pattern noise, and room lights, while keeping the Raman data in true spectral space. The cost-efficient design of the instrument allows rapid acquisition of shifted excitation data with a shift time penalty of less than 2 s. The Raman data are extracted from the shifted excitation spectra using a novel algorithm that is typically three orders of magnitude faster than conventional shifted-excitation algorithms operating in spectral space. The superiority of the instrument and algorithm in terms of background removal and signal-to-noise ratio is demonstrated by comparison to FT-Raman, standard deviation spectra, shifted excitation Raman difference spectroscopy (SERDS), and conventional multiple-shift excitation methods.