(*equal contribution; #corresponding authors) Ectopic expression of the cone-specific Gprotein-coupled receptor kinase GRK7 in zebrafish rods leads to decreased photosensitivity and compromised visual performance
(*equal contribution; #corresponding authors) Ectopic expression of the cone-specific Gprotein-coupled receptor kinase GRK7 in zebrafish rods leads to decreased photosensitivity and compromised visual performance
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(*同等贡献；
DOI：
10.1113/jphysiol.2010.204156
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Fukada,Y. & Lamb,T.
中科院分区：
文献类型：
--
作者：
Vogalis,F.;Shiraki,T.;Kojima,D.;Fukada,Y. & Lamb,T.
当眼睛中的视杆细胞和视锥细胞对光有反应时，它们需要恢复，而恢复的第一步涉及一种称为G蛋白受体激酶（GRK）的蛋白质。作为夜视基础的视杆细胞使用一种称为GRK1的变体，而调节白天视觉的视锥细胞通常使用一种称为GRK7的变体。我们已经在斑马鱼视网膜中设计了额外表达视锥细胞变体GRK7的视杆细胞。通过对这些经过修饰的视杆细胞进行电记录，我们发现它们对光的敏感性低于正常视杆细胞，在这方面，它们模仿了视锥细胞。我们还发现证据表明，当GRK1介导恢复时，细胞对单个光子（最小的光粒子）的反应大小是正常的，但当GRK7介导恢复时，细胞对单个光子（最小的光粒子）的反应大小很小（因此有点像锥形）。这些结果有助于我们理解视杆细胞和视锥细胞之间的差异。
Non‐technical summaryWhen rod and cone photoreceptors in the eye respond to light, they need to recover, and the first step in recovery involves a protein called G‐protein receptor kinase (GRK). Rods, which underlie night vision, employ a variant called GRK1, whereas cones, which mediate day vision, typically employ a variant called GRK7. We have engineered rod cells in the zebrafish retina that additionally express the cone variant, GRK7. By recording electrically from these modified rods, we have found that they are less sensitive to light than normal rods, in that regard mimicking cones. We have also found evidence to suggest that the size of the cell's response to a single photon (the smallest particle of light) is normal when recovery is mediated by GRK1, but is small (and hence somewhat cone‐like) when mediated by GRK7. These results help us understand the differences between rod and cone photoreceptors.