Mitochondrial characteristics of chicken breast muscle affected by wooden breast
Mitochondrial characteristics of chicken breast muscle affected by wooden breast
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DOI：
10.1016/j.cbpa.2022.111296
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发表时间：
2022-08-19
影响因子：
2.3
通讯作者：
Iwasaki, Tomohito
中科院分区：
文献类型：
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作者：
Hasegawa, Yasuhiro;Hosotani, Marina;Iwasaki, Tomohito
在过去的50年里，肉鸡的生长速度增加了400%，并且乳房产量持续增加。这导致肉鸡胸肌异常的增加，木胸成为世界范围内的主要问题。木乳房的病因和病因的机制尚未阐明;然而，它是由于氧化应激而发生的。引起氧化应激的活性氧主要在线粒体中产生。因此，在本研究中，我们探讨了肉仔鸡木胸的严重程度和线粒体作为活性氧来源的特性之间的关系。在50日龄肉仔鸡中进行腹侧颅位置处的胸大肌取样。根据肌纤维圆度和翼-翼接触试验将木胸的严重程度分为3组，严重木胸的严重程度最高，正常乳房的严重程度最低。烟酰胺腺嘌呤二核苷酸四唑还原酶染色显示深染肌纤维增加，表明木胸严重程度高。严重木乳病例的线粒体肿胀，严重木乳的肿胀最高，正常乳房的肿胀最低。线粒体抗氧化酶基因超氧化物歧化酶1和超氧化物歧化酶2的表达水平显着降低，在木乳房严重组织比正常组织。这些结果表明，当肌纤维中的活性氧水平（应该是恒定的）增加时，线粒体稳态不能维持，细胞各种膜的损伤水平增加，导致正常生理功能的破坏。
The growth rate of broiler chickens has increased by 400% over the past 50 years, and breast yields continue to increase. This has led to an increase in thoracic muscle abnormalities in broilers, with wooden breast becoming a major issue worldwide. The etiology and the mechanism underlying the etiology of wooden breasts have not yet been elucidated; however, it occurs due to oxidative stress. Reactive oxygen species, which cause oxidative stress, are mainly produced in mitochondria. Thus, in this study, we investigated the relationship between the severity of wooden breast in broilers and the characteristics of mitochondria as the source of reactive oxygen species. Sampling of the pectoralis major muscle at the ventral cranial position was conducted in 50-day-old broilers. The severity of wooden breast was classified into three groups based on the muscle fiber roundness and wing-wing contact test, with highest severity in severe wooden breast and lowest severity in normal breast. Nicotinamide adenine dinucleotide tetrazolium reductase staining revealed an increase in darkly stained muscle fibers, indicating high severity of wooden breast. The mitochondria were swollen in severe wooden breast cases, with highest swelling in severe wooden breast and lowest swelling in normal breast. The expression levels of the mitochondrial antioxidant enzyme genes superoxide dismutase 1 and superoxide dismutase 2 were significantly lower in wooden breast-severe tissue than in normal tissue. These results suggest that when the levels of reactive oxygen species in muscle fibers, which should be constant, are increased, mitochondrial homeostasis is not maintained and the damage levels increase in various membranes of the cell, leading to the disruption of normal physiological functions.