Visualizing the generation of memory CD4 T cells in the whole body
Visualizing the generation of memory CD4 T cells in the whole body
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DOI：
10.1038/35065111
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发表时间：
2001-03-01
期刊：
影响因子：
64.8
通讯作者：
Jenkins, MK
中科院分区：
文献类型：
--
作者：
Reinhardt, RL;Khoruts, A;Jenkins, MK
据认为，免疫依赖于幼稚的CD4 T细胞对微生物抗原的增殖反应（1-4），分化为产生抗微生物淋巴因子的记忆细胞（5,6），并迁移到感染部位（7,8）。在这里，我们使用免疫组织学枚举单个幼稚CD4 T细胞，特定于模型抗原，在成年小鼠的整个身体。这些细胞只存在于未暴露于抗原的小鼠的次级淋巴组织中，如脾脏和淋巴结。将抗原单独注射入血液后，T细胞增殖，迁移到肺、肝、肠和唾液腺，然后从这些器官中消失。如果将抗原与微生物产物脂多糖一起注射，增殖和迁移增强，两组记忆细胞存活数月：一组在淋巴结中产生生长因子白细胞介素-2，另一组在非淋巴组织中产生抗微生物淋巴因子干扰素- γ。这些结果表明，在感染的情况下，抗原识别产生记忆细胞，这些记忆细胞被专门用于在次级淋巴组织中增殖或在微生物进入的部位对抗感染。
It is thought that immunity depends on naive CD4 T cells that proliferate in response to microbial antigens(1-4), differentiate into memory cells that produce anti-microbial lymphokines(5,6), and migrate to sites of infection(7,8). Here we use immunohistology to enumerate individual naive CD4 T cells, specific for a model antigen, in the whole bodies of adult mice. The cells resided exclusively in secondary lymphoid tissues, such as the spleen and lymph nodes, in mice that were not exposed to antigen. After injection of antigen alone into the blood, the T cells proliferated, migrated to the lungs, liver, gut and salivary glands, and then disappeared from these organs. If antigen was injected with the microbial product lipopolysaccharide, proliferation and migration were enhanced, and two populations of memory cells survived for months: one in the lymph nodes that produced the growth factor interleukin-2, and a larger one in the non-lymphoid tissues that produced the anti-microbial lymphokine interferon-gamma. These results show that antigen recognition in the context of infection generates memory cells that are specialized to proliferate in the secondary lymphoid tissues or to fight infection at the site of microbial entry.