STORED AND POLYSOMAL RIBOSOMES OF MOUSE OVA
STORED AND POLYSOMAL RIBOSOMES OF MOUSE OVA
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DOI：
10.1016/0012-1606(77)90090-2
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发表时间：
1977-01-01
影响因子：
2.7
通讯作者：
DELEON, V
中科院分区：
文献类型：
--
作者：
BACHVAROVA, R;DELEON, V
通过将生长中的卵母细胞暴露于3 H-尿苷标记的排卵小鼠卵的RNP [核糖核蛋白]颗粒显示在蔗糖梯度上。在标准盐条件下，观察到放射性与肝脏核糖体亚基、单体和多核糖体一致。分离梯度每个区域的RNA，其中包含预期的标记18 S和/或28 S核糖体RNA。RNP颗粒在梯度中广泛分布。从RNase敏感性和高盐解离抗性的数据来看，总核糖体的20-25%在多聚核糖体中。在卵母细胞生长的早期或晚期，核糖体的分布没有差异。在poly（U）和蛋白质合成因子的存在下，非多聚体亚基和单体无法形成高盐稳定性的复合物。大部分的核糖体在排卵的卵子中蛋白质合成是不活跃的，显然是为了胚胎发育而储存的。
RNP [ribonuclear protein] particles of ovulated mouse ova, labeled by exposure of growing oocytes to 3H-uridine, were displayed on sucrose gradients. Under standard salt conditions, radioactivity was observed coinciding with liver ribosomal subunits, monomers and polysomes. The RNA from each region of the gradient was isolated and contained the expected species of labeled 18 S and/or 28 S ribosomal RNA. Heterogeneous RNP particles were widely distributed in the gradient. From data on RNase sensitivity and resistance to dissociation in high salt, 20-25% of the total ribosomes were in polysomes. No difference in the distribution was observed when ribosomes were labeled in the early or late growth phase of the oocyte. The nonpolysomal subunits and monomers were unable to form a high salt-stable complex in the presence of poly(U) and factors for protein synthesis. The bulk of the ribosomes were inactive in protein synthesis in ovulated ova and were apparently stored for use in embryonic development.