Development of Liposomal Nanoconstructs Targeting P-selectin (CD62P)-expressing Cells by Using A Sulfated Derivative of Sialic Acid
Development of Liposomal Nanoconstructs Targeting P-selectin (CD62P)-expressing Cells by Using A Sulfated Derivative of Sialic Acid
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DOI：
10.1007/s11095-014-1383-6
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发表时间：
2014-10-01
影响因子：
3.7
通讯作者：
Tsuji, Tsutomu
中科院分区：
文献类型：
--
作者：
Itoh, Saotomo;Kawano, Kumi;Tsuji, Tsutomu
目的NMSO 3是唾液酸的硫酸化衍生物，是P-选择素（CD 62 P）介导的细胞粘附的特异性抑制剂。方法荧光标记的NMSO 3脂质体（NMSO 3-liposomes）与表达P-选择素的CHO细胞（CHO-P细胞）和活化血小板的结合进行了研究。结果NMSO 3-脂质体与固定化的P-选择素和CHO-P细胞特异性结合，并呈剂量依赖性。NMSO_3-脂质体与CHO-P细胞的结合比与亲代CHOK_1细胞的结合强得多。荧光显微镜观察显示，NMSO 3-脂质体结合后进入CHO-P细胞，并分布在细胞的整个细胞质中。NMSO 3-脂质体结合更强烈的凝血酶活化的血小板比静息platelet.Conclusions通过流式细胞术评估这些结果表明，NMSO 3-脂质体可用于选择性药物输送到活化的血小板。
Purpose NMSO3, a sulfated derivative of sialic acid, is a specific inhibitor for P-selectin (CD62P)-mediated cell adhesion. We attempted to apply liposomes modified with NMSO3 for selective targeting of activated platelets.Methods The binding of fluorescently labeled NMSO3-containing liposomes (NMSO3-liposomes) to CHO cells expressing P-selectin (CHO-P cells) and activated platelets were examined. The distribution of NMSO3-liposomes incorporated into the cells was observed by fluorescence microscopy.Results The binding assay revealed that NMSO3-liposomes specifically bound to immobilized P-selectin and CHO-P cells in a dose-dependent manner. The binding of NMSO3-liposomes to CHO-P cells was much stronger than that to the parental CHOK1 cells. Fluorescence microscopic observation showed that NMSO3-liposomes were incorporated into CHO-P cells after the binding and distributed throughout the cytoplasm of the cell. NMSO3-liposomes bound more strongly to thrombin-activated platelets than to resting platelets, as assessed by flow cytometry.Conclusions These results suggest that NMSO3-liposomes can be applied for selective drug delivery to activated platelets.