Dietary fat composition influences glomerular and proximal convoluted tubule cell structure and autophagic processes in kidneys from calorie-restricted mice.
Dietary fat composition influences glomerular and proximal convoluted tubule cell structure and autophagic processes in kidneys from calorie-restricted mice.
复制标题
DOI：
10.1111/acel.12451
复制
发表时间：
2016-06
期刊：
影响因子：
7.8
通讯作者：
González-Reyes JA
中科院分区：
文献类型：
--
作者：
Calvo-Rubio M;Burón MI;López-Lluch G;Navas P;de Cabo R;Ramsey JJ;Villalba JM;González-Reyes JA
热量限制（CR）已多次被证明可以预防癌症，糖尿病，高血压和其他与年龄有关的疾病，在广泛的动物中，包括非人类灵长类动物和人类。在啮齿类动物中，CR还可以延长寿命，是研究衰老过程的有力工具。最近，据报道，在小鼠中，膳食脂肪在决定寿命延长方面起着重要作用，CR为40%。在这些条件下，与喂食大豆油或鱼油的小鼠相比，喂食猪油作为膳食脂肪的动物显示出更长的寿命。在本文中，我们研究了这些膳食脂肪对小鼠肾脏的结构和生理参数的影响，从保持在40%CR为6个月和18个月。使用定量电子显微镜技术和蛋白质印迹中的蛋白质表达进行分析。CR减轻了肾脏中分析的大部分年龄相关参数，如肾小球基底膜厚度、近曲小管中的线粒体质量和肾匀浆中的自噬标志物。与其他CR饮食组相比，猪油组显示出随着年龄的增长，几个肾脏结构的保护得到改善。这些结果表明，膳食脂肪调节CR小鼠的肾脏结构和功能，并在确定啮齿动物的健康寿命中起着重要作用。
Calorie restriction (CR) has been repeatedly shown to prevent cancer, diabetes, hypertension, and other age‐related diseases in a wide range of animals, including non‐human primates and humans. In rodents, CR also increases lifespan and is a powerful tool for studying the aging process. Recently, it has been reported in mice that dietary fat plays an important role in determining lifespan extension with 40% CR. In these conditions, animals fed lard as dietary fat showed an increased longevity compared with mice fed soybean or fish oils. In this paper, we study the effect of these dietary fats on structural and physiological parameters of kidney from mice maintained on 40% CR for 6 and 18 months. Analyses were performed using quantitative electron microcopy techniques and protein expression in Western blots. CR mitigated most of the analyzed age‐related parameters in kidney, such as glomerular basement membrane thickness, mitochondrial mass in convoluted proximal tubules and autophagic markers in renal homogenates. The lard group showed improved preservation of several renal structures with aging when compared to the other CR diet groups. These results indicate that dietary fat modulates renal structure and function in CR mice and plays an essential role in the determination of health span in rodents.