STUDIES ON LIPID-COMPOSITION OF RAT-LIVER ENDOPLASMIC-RETICULUM AFTER INDUCTION WITH PHENOBARBITONE AND 20-METHYLCHOLANTHRENE
STUDIES ON LIPID-COMPOSITION OF RAT-LIVER ENDOPLASMIC-RETICULUM AFTER INDUCTION WITH PHENOBARBITONE AND 20-METHYLCHOLANTHRENE
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DOI：
10.1042/bj1400461
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
WILLS, ED
中科院分区：
文献类型：
--
作者：
DAVISON, SC;WILLS, ED
1.单次注射20-甲基胆蒽或注射苯巴比妥5天后，研究大鼠肝内质网膜中胆固醇含量、磷脂酰胆碱和磷脂酰乙醇胺不同磷脂和脂肪酸成分的比例。 2.注射20-甲基胆蒽或苯巴比妥5天后，胆固醇比例明显下降。 3.注射苯巴比妥后磷脂酰胆碱比例增加，其他磷脂变化较小。 4.苯巴比妥使磷脂酰胆碱和磷脂酰乙醇胺中亚油酸的比例增加至对照的120%～125%，油酸、花生四烯酸和二十二碳六烯酸的比例下降。 5. 注射 1 天后，20-甲基胆蒽导致磷脂酰胆碱和乙醇胺中油酸的比例增加至对照的 125-140%。 6.苯巴比妥注射后磷脂酰胆碱中亚油酸比例的增加与细胞色素P-450浓度、氨基比林氧化脱甲基化速率和联苯羟基化速率的增加同时发生。因此，认为β位含有亚油酸的不同种类的磷脂酰胆碱或磷脂酰乙醇胺在内质网膜中对于氧化去甲基化的最佳活性至关重要。
1. The cholesterol content, proportions of different phospholipids and fatty acid components of phosphatidylcholine and phosphatidylethanolamine were studied in rat liver endoplasmic-reticulum membrane, after a single injection of 20-methylcholanthrene or injections of phenobarbitone for 5 days. 2. A marked decrease in the proportion of cholesterol occurred 5 days after injection of 20-methylcholanthrene or phenobarbitone. 3. The proportion of phosphatidylcholine was increased by injection of phenobarbitone and minor changes occurred in other phospholipids. 4. Phenobarbitone caused the proportion of linoleic acid in phosphatidylcholine and phosphatidylethanolamine to increase to 120–125% of the control and the proportion of oleic acid, arachidonic acid and docosahexaenoic acid to decrease. 5. 20-Methylcholanthrene caused an increase in the proportion of oleic acid in phosphatidylcholine and ethanolamine to 125–140% of the control, 1 day after injection. 6. The increased proportion of linoleic acid in phosphatidylcholine after phenobarbitone injection occurs simultaneously with the increase of cytochromeP-450 concentration, the rate of oxidative demethylation of aminopyrine and the rate of hydroxylation of biphenyl. It is therefore considered that distinct species of phosphatidylcholine or phosphatidylethanolamine containing linoleic acid in the β position are essential in the endoplasmic-reticulum membrane for optimal activity of oxidative demethylation.