Measuring mitochondrial shape changes and their consequences on mitochondrial involvement during apoptosis.
Measuring mitochondrial shape changes and their consequences on mitochondrial involvement during apoptosis.
复制标题
DOI：
10.1007/978-1-59745-365-3_29
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Scorrano, Luca
中科院分区：
文献类型：
--
作者：
Frezza, Christian;Cipolat, Sara;Scorrano, Luca
线粒体是细胞内部和某些细胞类型外部刺激后死亡的关键参与者。线粒体途径的募集导致线粒体功能障碍和释放膜间空间蛋白，如细胞色素-c，这是细胞质中完全激活效应半胱天冬酶所必需的。这种细胞器的凋亡形状变化和“线粒体成形”蛋白在细胞死亡中的作用引起了相当大的关注。我们提出了研究线粒体网络形态变化如何调节细胞色素c释放的方案，通过原位方法定量评估，并实时测量线粒体膜电位的变化。
Mitochondria are key players in cell death following intrinsic and, in some cell types, extrinsic stimuli. The recruitment of the mitochondrial pathway results in mitochondrial dysfunction and release of intermembrane space proteins like cytochrome-c that are required in the cytosol for complete activation of effector caspases. Apoptotic shape changes of this organelle and the role of "mitochondria-shaping" proteins in cell death has attracted considerable attention. We present protocols to investigate how morphological changes of the mitochondrial reticulum regulate release of cytochrome-c, as evaluated quantitatively by an in situ approach, and changes in mitochondrial membrane potential measured in real time.