Prostaglandin F2α induces a rapid decline in progesterone production and steroidogenic acute regulatory protein expression in isolated rat corpus luteum without altering messenger ribonucleic acid expression
Prostaglandin F2α induces a rapid decline in progesterone production and steroidogenic acute regulatory protein expression in isolated rat corpus luteum without altering messenger ribonucleic acid expression
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DOI：
10.1095/biolreprod61.3.643
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发表时间：
1999-09-01
影响因子：
3.6
通讯作者：
Khan, I
中科院分区：
文献类型：
--
作者：
Fiedler, EP;Plouffe, L;Khan, I
由于对类固醇生成急性调节蛋白（星星）参与黄体溶解过程的兴趣，我们研究了血清孕酮水平的变化以及星星mRNA和蛋白的伴随表达。注射前列腺素F-2 α后1小时，从大鼠中分离的排卵后第7天黄体（CL）中的37、32和30 kDa形式采用放射免疫分析法测定血清孕酮水平，北方分析法测定StAR和β-actin mRNA表达，Western分析法测定星星和β-actin蛋白表达。来自对照动物的肾上腺、脑和脾用作星星表达的阳性和阴性对照。扫描光密度测定法测量值通过将来自每个星星放射自显影泳道的信号强度除以来自相应的β-肌动蛋白放射自显影泳道的信号强度来标准化。ANOVA用于显著性检验，α设定为0.05。星星蛋白的37-、32-和30-kDa形式在所有肾上腺样品中表达，而在CL中仅发现37-和30-kDa形式。发现在PGF（2 α）处理组中，血清孕酮水平以及CL中星星蛋白的30-kDa和37-kDa形式的表达均显著低于盐水处理组。星星mRNA表达在生理盐水和PGF（2 α）处理的大鼠中没有显著差异。因此，伴随PGF（2 α）诱导的黄体溶解的星星蛋白表达的快速下降不是mRNA表达显著下降的结果。
With interest in steroidogenic acute regulatory protein (StAR) involvement in the luteolytic process, we studied changes in serum progesterone levels and the concomitant expression of StAR mRNA and protein (37-, 32-, and 30-kDa forms) in postovulatory Day 7 corpora lutea (CL) isolated from rats 1 h after injection with prostaglandin F-2 alpha (PCF2 alpha, n = 6) or saline (n = 6), Serum progesterone levels were determined by RIA, StAR and beta-actin mRNA expression by Northern analysis, and StAR and beta-actin protein expression by Western analysis. Adrenal, brain, and spleen from control animals were used as positive and negative controls for StAR expression. Scanning optical densitometry measurements were standardized by dividing the signal strength from each StAR autoradiogram lane by that from the corresponding beta-actin autoradiogram lane. ANOVA was used for significance testing, with alpha set at 0.05. The 37-, 32-, and 30-kDa forms of StAR protein were expressed in all adrenal samples, whereas only the 37- and 30-kDa forms were found in CL, Serum progesterone levels and expression of the 30-kDa and 37-kDa forms of the StAR protein in CL were all found to be significantly lower in the PGF(2 alpha)-treated than the saline-treated group. StAR mRNA expression was not significantly different in the saline- and PGF(2 alpha)-treated rats. The rapid decline in StAR protein expression that accompanies PGF(2 alpha) induced luteolysis, therefore, does not result from significant decline in mRNA expression.