Aberrant Synthesis of Indole-3-Acetic Acid in Saccharomyces cerevisiae Triggers Morphogenic Transition, a Virulence Trait of Pathogenic Fungi
Aberrant Synthesis of Indole-3-Acetic Acid in Saccharomyces cerevisiae Triggers Morphogenic Transition, a Virulence Trait of Pathogenic Fungi
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DOI：
10.1534/genetics.109.112854
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发表时间：
2010-05-01
期刊：
影响因子：
3.3
通讯作者：
Normanly, Jennifer
中科院分区：
文献类型：
--
作者：
Rao, Reeta Prusty;Hunter, Ally;Normanly, Jennifer
许多与植物相关的微生物合成生长素吲哚-3-乙酸(IAA)，在微生物和植物中已经发现了几种IAA的生物合成途径。酿酒酵母此前已被证明通过诱导假菌丝生长来响应IAA。我们观察到，IAA还诱导了人类病原体白色念珠菌的菌丝生长，因此可能作为次生代谢产物信号调节致病真菌的毒力特征，如菌丝过渡。在玉米黑粉菌中，从色氨酸(Trp)前体合成IAA需要乙醛脱氢酶(Ald)。两个ALD基因缺失的突变酿酒酵母不能将放射性标记的Trp转化为IAA，但在没有外源Trp的情况下仍能产生IAA，且水平高于野生型。这些数据表明，酵母可能有多条合成IAA的途径，其中一条不依赖于色氨酸。
Many plant-associated microbes synthesize the auxin indole-3-acetic acid (IAA), and several IAA biosynthetic pathways have been identified in microbes and plants. Saccharomyces cerevisiae has previously been shown to respond to IAA by inducing pseudohyphal growth. We observed that IAA also induced hyphal growth in the human pathogen Candida albicans and thus may function as a secondary metabolite signal that regulates virulence traits such as hyphal transition in pathogenic fungi. Aldehyde dehydrogenase (Ald) is required for IAA synthesis from a tryptophan (Trp) precursor in Ustilago maydis. Mutant S. cerevisiae with deletions in two ALD genes are unable to convert radiolabeled Trp to IAA, yet produce IAA in the absence of exogenous Trp and at levels higher than wild type. These data suggest that yeast may have multiple pathways for IAA synthesis, one of which is not dependent on Trp.