Difference in organization of corticostriatal and thalamostriatal synapses between patch and matrix compartments of rat neostriatum
Difference in organization of corticostriatal and thalamostriatal synapses between patch and matrix compartments of rat neostriatum
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DOI：
10.1111/j.1460-9568.2006.05177.x
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发表时间：
2006-11-01
影响因子：
3.4
通讯作者：
Kaneko, Takeshi
中科院分区：
文献类型：
--
作者：
Fujiyama, Fumino;Unzai, Tomo;Kaneko, Takeshi
新纹状体具有由斑片和基质隔室组成的镶嵌组织，接受来自大脑皮层和丘脑的多巴胺能兴奋性传入。这些纹状体传入之间的补丁和基质室的突触组织的差异进行了检查，在大鼠使用共聚焦激光扫描和电子显微镜。囊泡谷氨酸转运体（VGluT）2的丘脑纹状体终端免疫阳性的补丁密度小于在基质室，虽然VGluT1免疫阳性皮质纹状体终端的密度几乎均匀分布在两个车厢。超微结构图像的定量分析表明，84%的VGluT2阳性突触的补丁室形成的树突棘，而70%的基质室与树突轴。相比之下，VGluT1阳性终端显示出类似的偏好，在两个隔间的特定的突触目标：约80%的突触与树突棘。此外，VGluT2阳性轴棘突触在补丁隔室大于VGluT1阳性轴棘突触在两个隔室。由于轴棘突触通常存在于表现出高突触可塑性的神经元连接中，因此本研究结果表明，丘脑纹状体连接在斑块隔室中比在基质隔室中需要更高的突触可塑性。
The neostriatum, which possesses a mosaic organization consisting of patch and matrix compartments, receives glutamatergic excitatory afferents from the cerebral cortex and thalamus. Differences in the synaptic organization of these striatopetal afferents between the patch and matrix compartments were examined in the rat using confocal laser scanning and electron microscopes. Thalamostriatal terminals immunopositive for vesicular glutamate transporter (VGluT) 2 were less dense in the patch than in the matrix compartment, although the density of VGluT1-immunopositive corticostriatal terminals was almost evenly distributed in both the compartments. Quantitative analysis of ultrastructural images revealed that 84% of VGluT2-positive synapses in the patch compartment were formed with dendritic spines, whereas 70% in the matrix compartment were made with dendritic shafts. By contrast, VGluT1-positive terminals display a similar preference for specific synaptic targets in both compartments: about 80% made synapses with dendritic spines. In addition, VGluT2-positive axospinous synapses in the patch compartment were larger than the VGluT1-positive axospinous synapses in both compartments. As axospinous synapses are generally found in neuronal connections showing high synaptic plasticity, the present findings suggest that the thalamostriatal connection requires higher synaptic plasticity in the patch compartment than in the matrix compartment.