Mild Staphylococcus aureus Skin Infection Improves the Course of Subsequent Endogenous S. aureus Bacteremia in Mice
Mild Staphylococcus aureus Skin Infection Improves the Course of Subsequent Endogenous S. aureus Bacteremia in Mice
复制标题
轻度金黄色葡萄球菌皮肤感染可改善小鼠随后内源性金黄色葡萄球菌菌血症的病程
作者：
S. van den Berg;C. D. de Vogel;A. van Belkum;I. Bakker
与非携带者相比，患有金黄色葡萄球菌菌血症的金黄色葡萄球菌携带者的死亡风险可能降低。建议免疫系统发挥作用。在这里，我们研究了小鼠在内源性或外源性金黄色葡萄球菌菌血症之前轻度金黄色葡萄球菌皮肤感染的影响，并评估与抗葡萄球菌抗体水平相关的保护作用。在不含金黄色葡萄球菌和抗葡萄球菌抗体的小鼠中，诱导临床金黄色葡萄球菌分离株（分离株 P）或金黄色葡萄球菌菌株 8325-4 引起一次或两次皮肤感染。五周后，测定血液中针对25种金黄色葡萄球菌抗原的免疫球蛋白G（IgG）水平，并诱导由金黄色葡萄球菌分离株P引起的LD50或LD100菌血症。金黄色葡萄球菌皮肤感染导致血液中抗葡萄球菌 IgG 水平升高。一种皮肤感染仅改善了随后的严重内源性菌血症的病程。第二次皮肤感染进一步提高了动物的存活率，这与针对 Efb、IsaA、LukD、LukE、Nuc、PrsA 和 WTA 的菌血症前 IgG 水平升高有关。总之，小鼠金黄色葡萄球菌分离株 P 皮肤感染仅降低随后的内源性金黄色葡萄球菌菌血症的严重程度。虽然不能排除细胞免疫效应，但针对特定抗原的抗葡萄球菌 IgG 可能有助于这种效应。
Staphylococcus aureus carriers with S. aureus bacteremia may have a reduced mortality risk compared to non-carriers. A role for the immune system is suggested. Here, we study in mice the effect of mild S. aureus skin infection prior to endogenous or exogenous S. aureus bacteremia, and evaluate protection in relation to anti-staphylococcal antibody levels. Skin infections once or twice by a clinical S. aureus isolate (isolate P) or S. aureus strain 8325-4 were induced in mice free of S. aureus and anti-staphylococcal antibodies. Five weeks later, immunoglobulin G (IgG) levels in blood against 25 S. aureus antigens were determined, and LD50 or LD100 bacteremia caused by S. aureus isolate P was induced. S. aureus skin infections led to elevated levels of anti-staphylococcal IgG in blood. One skin infection improved the course of subsequent severe endogenous bacteremia only. A second skin infection further improved animal survival rate, which was associated with increased pre-bacteremia IgG levels against Efb, IsaA, LukD, LukE, Nuc, PrsA and WTA. In conclusion, S. aureus isolate P skin infection in mice reduces the severity of subsequent endogenous S. aureus bacteremia only. Although cellular immune effects cannot be rules out, anti-staphylococcal IgG against specified antigens may contribute to this effect.
影响因子：
3.4
作者：
Ziebandt, Anne-Kathrin;Kusch, Harald;Engelmann, Susanne
通讯作者：
Engelmann, Susanne