Post-weaning social isolation rearing influences the expression of molecules related to inhibitory neurotransmission and structural plasticity in the amygdala of adult rats
Post-weaning social isolation rearing influences the expression of molecules related to inhibitory neurotransmission and structural plasticity in the amygdala of adult rats
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DOI：
10.1016/j.brainres.2012.01.073
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发表时间：
2012-04-11
期刊：
影响因子：
2.9
通讯作者：
Nacher, Juan
中科院分区：
文献类型：
--
作者：
Gilabert-Juan, Javier;Dolores Molto, Maria;Nacher, Juan
几条线索的证据表明，神经回路和抑制性神经传递的结构的改变是精神分裂症的病理生理机制的基础。大多数关于这些参数的研究都集中在皮层区域，尽管杏仁核在这种精神疾病中起着至关重要的作用，但关于该区域的信息较少。为了扩大这方面的知识，我们研究了与抑制性神经传递和结构可塑性相关的分子在断奶后隔离饲养的大鼠中的表达，这种动物模型再现了精神分裂症的几种核心症状。我们已经使用qRT-PCR和免疫组织化学分析了突触素、GAD 65、GAD 67、神经细胞粘附分子（NCAM）、其聚唾液酸化形式（PSA-NCAM）及其合成酶（St 8 siaII和St 8 SiaIV）的表达。隔离饲养的大鼠表现出显着增加的GAD 67蛋白的表达在中央，内侧和基底外侧杏仁核，但没有显着变化GAD 65或突触素的表达被发现在这些地区。PSA-NCAM和NCAM在基底外侧核和内侧核的表达分别显著增加。我们的研究结果表明，隔离饲养积极影响抑制性神经传递和杏仁核神经元的结构可塑性，可能通过PSA-NCAM。这些发现与报道精神分裂症患者杏仁核中抑制性神经传递相关分子表达减少相反。因此，尽管社会孤立的摇晃模型可以重现精神分裂症患者的某些行为特征，但它可能无法重现这种疾病的某些神经生物学特征，特别是在杏仁核中。(C)2012爱思唯尔有限公司版权所有。
Several lines of evidence indicate that alterations in the structure of neural circuits and inhibitory neurotransmission underlie the physiopathogenesis of schizophrenia. Most of the studies on these parameters have been focused on cortical regions and, despite the crucial role of the amygdala in this psychiatric disorder, there is less information on this region. In order to expand this knowledge, we have studied the expression of molecules related to inhibitory neurotransmission and structural plasticity in rats subjected to post-weaning isolation rearing, an animal model that reproduces several core symptoms of schizophrenia. We have analyzed, using qRT-PCR and immunohistochemistry, the expression of synaptophysin, GAD65, GAD67, the neural cell adhesion molecule (NCAM), its polysialylated form (PSA-NCAM) and its synthesizing enzymes (St8siaII and St8SiaIV). Isolation-reared rats showed significant increases in the expression of GAD67 protein in the centromedial, medial and basolateral amygdaloid nuclei, but no significant changes in GAD65 or synaptophysin expression were found in these regions. The expression of PSA-NCAM and NCAM was significantly increased in the basolateral and medial nuclei respectively. Our results indicate that isolation-rearing influences positively inhibitory neurotransmission and neuronal structural plasticity in the amygdala, probably through PSA-NCAM. These findings are in contrast to reports describing decreased expression of molecules related to inhibitory neurotransmission in the amygdala of schizophrenic patients. Consequently, although the social isolation reeling model can reproduce some of the behavioral traits of schizophrenics it may fail to reproduce some of the neurobiological features of this disorder, particularly in the amygdala. (C) 2012 Elsevier B.V. All rights reserved.