Stem cell properties of human dental pulp stem cells
Stem cell properties of human dental pulp stem cells
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DOI：
10.1177/154405910208100806
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发表时间：
2002-08-01
影响因子：
7.6
通讯作者：
Shi, S
中科院分区：
文献类型：
--
作者：
Gronthos, S;Brahim, J;Shi, S
在这项研究中，我们的特点是自我更新能力，多谱系分化能力，和克隆形成效率的人牙髓干细胞（DPSCs）。DPSCs能够在体内形成异位牙本质和相关的牙髓组织。基质样细胞从原代DPSC移植物培养中重建，并重新移植到免疫功能低下的小鼠中，以产生牙本质牙髓样组织，证明其自我更新能力。DPSC还被发现能够分化为脂肪细胞和神经样细胞。12个单独的单菌落衍生的DPSC菌株的牙源性潜力进行了测定。三分之二的单菌落衍生的DPSC菌株在体内产生丰富的异位牙本质，而在剩下的三分之一中仅检测到有限量的牙本质。这些结果表明，单菌落衍生的DPSC菌株彼此不同，就其牙发生率。总之，这些结果表明，DPSC具有干细胞样的品质，包括自我更新能力和多谱系分化。
In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.