Heterologous leaky production of transglutaminase in Lactococcus lactis significantly enhances the growth performance of the host
Heterologous leaky production of transglutaminase in Lactococcus lactis significantly enhances the growth performance of the host
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DOI：
10.1128/aem.71.12.8911-8919.2005
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Li, Y
中科院分区：
文献类型：
--
作者：
Fu, RY;Chen, J;Li, Y
这项研究描述了一种通过异源生产食品级转谷氨酰胺酶来改善乳酸乳球菌生长性能的新策略。将来自茂原链轮丝菌的编码转谷氨酰胺酶成熟蛋白的 mtg 基因克隆到乳链菌肽诱导型表达载体中，并转化到乳酸乳球菌亚种中。乳脂 NZ9000。 nisA 启动子的 mtg 基因的泄漏表达导致丙酮酸分支处氨的形成和碳通量的重新分配。结果，减少了介质酸化并提高了能源利用率。这导致在有氧条件下，特别是在非 pH 控制条件下，生物量产量显着提高（最多增加 12 倍）。这里提出的结果提供了一种提高乳酸乳球菌生长产量的新方法，这是使用乳酸乳球菌作为宿主生产具有商业价值的蛋白质的重要一步。
This study describes a novel strategy to improve the growth performance of Lactococcus lactis by heterologous production of food-grade transglutaminase. The mtg gene from Streptoverticillium mobaraense that encodes the transglutaminase mature protein was cloned into a nisin-inducible expression vector and transformed into L. lactis subsp. cremoris NZ9000. The leaky expression of the mtg gene from the nisA promoter resulted in ammonia formation and carbon flux redistribution at the pyruvate branch. As a consequence, medium acidification was lessened and energy utilization was improved. This led to significantly higher biomass production under aerobic conditions and particularly under non-pH-controlled conditions (up to a 12-fold increase). The results presented here provide a novel way to enhance the growth yield of L. lactis, which is an important step for the purposes of producing proteins of commercial interest using L. lactis as a host.