Quantitative analyses of leukemia inhibitory factor in the cerebrospinal fluid in mouse embryos
Quantitative analyses of leukemia inhibitory factor in the cerebrospinal fluid in mouse embryos
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DOI：
10.1097/wnr.0b013e3280113962
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发表时间：
2006-12-18
期刊：
影响因子：
1.7
通讯作者：
Otani, Hiroki
中科院分区：
文献类型：
--
作者：
Hatta, Toshihisa;Matsumoto, Akihiro;Otani, Hiroki
白血病抑制因子有助于前脑神经干细胞的自我更新。虽然脑实质中是否存在内源性白血病抑制因子一直存在争议，但已知脑脊液是白血病抑制因子的另一个来源。然而，目前还没有关于脑脊液中白血病抑制因子浓度测量的报道。本研究采用酶联免疫吸附试验测定了小鼠脑脊液、羊水、胚胎和母鼠血清中白血病抑制因子的浓度。白血病抑制因子在胚胎第11天至第17天的脑脊液中呈结构性高浓度，在第13天和第14天达到高峰。这些结果与小鼠大脑皮质神经元产生的时间相一致。
Leukemia inhibitory factor contributes to the self-renewal of neural stem cells in the forebrain. Although the existence of endogenous leukemia inhibitory factor in the brain parenchyma has been controversial, the cerebrospinal fluid is known to be another source of leukemia inhibitory factor. No reports of the measurement of leukemia inhibitory factor concentrations in the cerebrospinal fluid, however, exist. In the present study, we determined the leukemia inhibitory factor concentration in cerebrospinal fluid, amniotic fluid, and sera of embryos and dams in mice by enzyme-linked immunosorbent assay. The leukemia inhibitory factor concentrations were found to be constitutively high in the cerebrospinal fluid from embryonic day 11 to embryonic day 17, with a peak on embryonic day 13 and embryonic day 14. These findings correspond to the timing of cortical neuron production in mouse cerebrum.