Green seaweed Enteromorpha compressa (Chlorophyta, Ulvaceae) derived sulphated polysaccharides inhibit herpes simplex virus
Green seaweed Enteromorpha compressa (Chlorophyta, Ulvaceae) derived sulphated polysaccharides inhibit herpes simplex virus
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DOI：
10.1016/j.ijbiomac.2017.04.043
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发表时间：
2017-09-01
影响因子：
8.2
通讯作者：
Nozawa, Carlos
中科院分区：
文献类型：
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作者：
Lopes, Nayara;Ray, Sayani;Nozawa, Carlos
单纯疱疹病毒（HSV）疾病由于其传播性和潜伏期后的复发性，代表了一个相关的医疗和社会问题。绿色藻类是被称为石莼的多糖的丰富来源，据报道具有生物活性和抗真菌活性。本研究分析了七种经化学修饰的浒苔多糖对HSV的抑制活性。只有命名为SU 1F 1的衍生物显示出令人满意的病毒抑制活性，具有高选择性指数，因此，对其进行了可能的作用机制和结构分析。SU 1F 1是一种硫酸化（22% w/w）杂糖醛酸，表观分子量为34 kDa。采用空斑减数法，以加入时间（感染前3 h至感染后16 h）、病毒吸附和穿透抑制率以及杀病毒效果为指标，测定其抗病毒活性。SU 1F 1在当时显示出高病毒活性。我们证明，其抑制作用维持至处理后4小时，在100 μ g/ml下具有100%的病毒抑制。在其他方案（预处理、吸附和渗透抑制以及杀病毒试验）中未观察到任何影响。逆转录酶相关PCR（RT-PCR）结果与空斑减少试验一致，并证明了SU 1F 1在HSV复制的初始阶段的活性。(C)2017爱思唯尔B. V.保留所有权利。
The herpes simplex virus (HSV) diseases represent a relevant medical and social problem due to their communicability and recurrence following latency. The green algae are rich source of polysaccharides referred to as ulvans, reported as being biologically and pharmacologically active. In this work, we analyzed the activity of seven chemically modified polysaccharides from Enteromorpha compressa (Chlorophyta, Ulvaceae), against HSV. Only the derivative named SU1F1 showed satisfactory viral inhibition activity, with a high selectivity index, and, therefore, it was submitted to analysis of the probable mechanism of action and structure. SU1F1 is a sulphated (22% w/w) heteroglycuronan with an apparent molecular mass of 34 kDa. The antiviral activity was assayed by plaque reduction assay under the protocols of the time-of-addition (from 3 h before infection to 16 h after infection), the inhibition of virus adsorption and penetration, and the virucidal effects. SU1F1 showed a high viral activity at the time Oh. We demonstrated that its inhibitory effect was maintained until 4h post-treatment with 100% of viral inhibition at 100 mu g/ml. No effect was observed in additional protocols (the pre-treatment, the inhibition of adsorption and penetration and virucidal assays). Reverse Transcriptase associated PCR (RT-PCR) results were in accordance with plaque reduction assay and demonstrated the activity of SU1F1 at the initial stages of HSV replication. (C) 2017 Elsevier B.V. All rights reserved.