Flavonoids, a Potential New Insight of Leucaena leucocephala Foliage in Ruminant Health
Flavonoids, a Potential New Insight of Leucaena leucocephala Foliage in Ruminant Health
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黄酮类化合物，银合欢叶子对反刍动物健康的潜在新见解
DOI：
10.1021/acs.jafc.8b02739
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发表时间：
2018
影响因子：
6.1
通讯作者：
Zhou ZY
中科院分区：
文献类型：
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作者：
Xu Yingchao;Tao Zhenru;Jin Yu;Yuan Yunfei;Zhou Zhongyu;Doug Tina T. X.;Tsim Karl W. K.;Doug Tina T. X.;Tsim Karl W. K.;Xu Yingchao;Tao Zhenru;Jin Yu;Zhou ZY
对采自广东中国的银合欢的化学成分进行了研究，分离出17种不同的黄酮类化合物(1-17)，包括黄酮类(5-9、11和12)、黄酮醇(1、10和16)、黄酮酮4、黄酮醇15和黄酮苷(2、3、13、14和17)。黄酮类成分主要为槲皮素(1)、3-O-α-鼠李糖苷(2)和杨梅素-3-O-α-鼠李糖苷(17)。白头叶，总浓度约为干物质的2.5%。对模拟促红细胞生成素(EPO)基因激活的Phre-Luc诱导活性、抗炎、抗糖尿病和抗氧化活性进行了评价。黄酮类化合物7、10和13能够强烈地诱导Phre-Luc的转录活性，这表明它们有可能诱导EPO的表达。黄酮类化合物7、10、13和17具有较强的抗炎活性，与阳性对照地塞米松相当。黄酮类化合物1、2、3、11、12、16和17清除DPPH自由基的能力强于抗坏血酸。黄酮类化合物1、2和10对叔丁基氢过氧化氢(TBHP)诱导的ROS形成具有较弱的细胞抗氧化活性。黄酮类鼠李糖苷2和阿拉伯糖苷3在牛瘤胃微生物厌氧培养条件下脱糖生成苷元槲皮素。此外，富含INL的类黄酮类化合物对反刍动物具有潜在的健康益处。文中还讨论了脑白质疏松。
We investigated the constituents ofLeucaena leucocephalafoliage collected from Guangdong province in China and isolated 17 diverse flavonoids (1–17), including flavones (5–9,11, and12), flavonols (1,10, and16), flavanone4, flavanonol15, and flavonol glycosides (2,3,13,14, and17). Flavonoids quercetin (1), quercetin-3-O-α-rhamnopyranoside (2), and myricetin-3-O-α-rhamnopyranoside (17) were the major flavonoids components inL. leucocephalaleaves, at a total concentration of about 2.5% of dry matter. pHRE-Luc inductive activity to mimic the activation of erythropoietin (EPO) gene, anti-inflammatory, antidiabetic, and antioxidant activities of isolated flavonoids (1–17) were evaluated. Flavonoids7,10, and13could strongly induce the transcriptional activity of pHRE-Luc, which indicated their potential to induce the expression of EPO. Flavonoids7,10,13, and17displayed strong anti-inflammatory activity, relatively equal to the positive control dexamethasone. Flavonoids1,2,3,11,12,16, and17showed stronger antioxidant activities of DPPH radical scavenging capacity than ascorbic acid. Flavonoids1,2, and10showed weak cellular antioxidant activities againsttert-butyl hydroperoxide (tBHP) induced ROS formation. Flavonoid rhamnoside2and arabinoside3undergone deglycosylation to the aglycone quercetin under anaerobic incubation with cattle rumen microorganisms. Furthermore, the potential health benefits for ruminant of flavonoids, which was rich inL. leucocephalafoliage, was also discussed.