In vitro parasite-monocyte interactions in human leishmaniasis: possible role of fibronectin in parasite attachment.
In vitro parasite-monocyte interactions in human leishmaniasis: possible role of fibronectin in parasite attachment.
复制标题
人类利什曼病的体外寄生虫-单核细胞相互作用：纤连蛋白在寄生虫附着中的可能作用。
DOI：
10.1128/iai.49.2.305-311.1985
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
McDonald,JA
中科院分区：
文献类型：
--
作者：
Wyler,DJ;Sypek,JP;McDonald,JA
Leishmania spp. must attach to mononuclear phagocyte surfaces before entering this host cell. We investigated the potential role of fibronectin in facilitating parasite attachment. Human plasma fibronectin bound to axenically cultured promastigotes, and promastigotes and amastigotes preferentially bound to fibronectin-coated cover slips. Promastigotes grown in the absence of fibronectin were strikingly deficient in their ability to attach to human monocytes compared with promastigotes grown in the presence of fibronectin. Rabbit anti-human plasma fibronectin antiserum decreased promastigote and amastigote attachment to monocytes. Immunoglobulin G F(ab')2 and Fab fragments also reduced the ability of amastigotes to bind to monocytes. Antiserum pretreatment of amastigotes followed by washing resulted in reduced parasite binding, whereas antibody pretreatment of monocytes did not. Addition of exogenous fibronectin did not enhance parasite attachment to monocytes. These findings suggest that Leishmania spp. can bind fibronectin and may utilize this glycoprotein to facilitate attachment to the mononuclear phagocytes that they infect.
