The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the, spindle checkpoint
The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the, spindle checkpoint
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DOI：
10.1101/gad.934801
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发表时间：
2001-12-01
影响因子：
10.5
通讯作者：
Murray, AW
中科院分区：
文献类型：
--
作者：
Biggins, S;Murray, AW
纺锤体检查点阻止具有有丝分裂纺锤体缺陷的细胞中的细胞周期进展。虽然一些纺锤体缺陷激活纺锤体检查点，主要信号的确切性质是未知的。我们已经发现，极光蛋白激酶家族的芽殖酵母成员Ipl 1 p是维持纺锤体检查点停滞的一个子集所必需的。IPL 1 P是。这是维持由蛋白激酶Mps 1过表达诱导的纺锤体检查点所必需的。使Ipl 1 p失活允许过表达Mps 1 p的细胞逃避有丝分裂并正常分离其染色体。因此，纺锤体检查点中对Ipl 1 p的需求不是动粒和/或纺锤体缺陷的结果。对Ipl 1 p的需求区分了纺锤体检查点的两种不同激活剂：Ipl 1 p功能是由其动粒不处于张力下的染色体触发的延迟所需的，但不是由纺锤体解聚诱导的停滞所需的。Ipl 1 p定位在或附近的动粒在有丝分裂过程中，我们建议，Ipl 1 p是需要监测紧张的动粒。
The spindle checkpoint prevents cell cycle progression in cells that have mitotic spindle defects. Although several spindle defects activate the spindle checkpoint, the exact nature of the primary signal is unknown. We have found that the budding yeast member of the Aurora protein kinase family, Ipl1p, is required to maintain a subset of spindle checkpoint arrests., Ipl1p is. required to maintain the spindle checkpoint that is induced by overexpression of the protein kinase Mps1. Inactivating Ipl1p allows cells overexpressing Mps1p to escape from mitosis and segregate their chromosomes normally. Therefore, the requirement for Ipl1p in the spindle checkpoint is not a consequence of kinetochore and/or spindle defects. The requirement for Ipl1p distinguishes two different activators of the spindle checkpoint: Ipl1p function is required for the delay triggered by chromosomes whose kinetochores are not under tension, but is not required for arrest induced by spindle depolymerization. Ipl1p localizes at or near kinetochores during mitosis, and we propose that Ipl1p is required to monitor tension at the kinetochore.